Fascin Antibody (JM12-53)

Western Blot: Fascin Antibody (JM12-53) [NBP2-66832] - Analysis of Fascin on different lysates using anti-Fascin antibody at 1/1,000 dilution. Positive control: Lane1: SH-SY5Y Lane2: Human brain Lane3: Rat spleen Lane4: Mouse testes Lane5: Mouse brain Lane6: Mouse spleen

Immunocytochemistry/Immunofluorescence: Fascin Antibody (JM12-53) [NBP2-66832] - Staining Fascin in SW480 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: Fascin Antibody (JM12-53) [NBP2-66832] - Analysis of paraffin-embedded rat brain tissue using anti-Fascin antibody. Counter stained with hematoxylin.

Flow Cytometry: Fascin Antibody (JM12-53) [NBP2-66832] - Analysis of Hela cells with Fascin antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).

Immunocytochemistry/Immunofluorescence: Fascin Antibody (JM12-53) [NBP2-66832] - Staining Fascin in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunocytochemistry/Immunofluorescence: Fascin Antibody (JM12-53) [NBP2-66832] - Staining Fascin in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: Fascin Antibody (JM12-53) [NBP2-66832] - Analysis of paraffin-embedded human kidney tissue using anti-Fascin antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: Fascin Antibody (JM12-53) [NBP2-66832] - Analysis of paraffin-embedded human tonsil tissue using anti-Fascin antibody. Counter stained with hematoxylin.

Product Discontinued

Fascin Antibody (JM12-53) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Recombinant protein within Human Fascin aa 221-389 / 493. (SwissProt: Q16658 Human; SwissProt: Q61553 Mouse; SwissProt: P85845 Rat)
• Localization: Cytoskeleton, stress fiber, cytosol, cell cortex, filopodium, invadopodium, microvillus, cell junction.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: FSCN1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Immunoprecipitation
  • Western Blot 1:500-1:2000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for Fascin Antibody (JM12-53)

• FAN1
• FAN1,55 kDa actin-bundling protein
• fascin homolog 1, actin-bundling protein (Strongylocentrotus purpuratus)
• Fascin
• FLJ38511
• FSCN1
• HSN
• p55actin bundling protein
• singed (Drosophila)-like (sea urchin fascin homolog like)
• singed-like (fascin homolog, sea urchin)
• Singed-like Protein
• SNL
• SNLfascin

Background

Organizes filamentous actin into bundles with a minimum of 4.1:1 actin/fascin ratio. Probably involved in the assembly of actin filament bundles present in microspikes, membrane ruffles, and stress fibers

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: FSCN1