FANCI Knockout 293T Cell Lysate

Product Discontinued

FANCI Knockout 293T Cell Lysate Summary

• Preparation Method: Knockout achieved by using CRISPR/Cas9,2 bp deletion in exon 4 and Insertion of the selection cassette in exon 4
• Gene: FANCI

Applications/Dilutions

• Dilutions:
  • Western Blot
• Application Notes: You will receive 1 vial (100ug) of knockout cell lysate and 1 vial (100ug) of Parental cell lysate. Lysate can be diluted with 1X SDS sample buffer and will be stable at -20 degrees C for 12 months. Minimize freeze-thaw cycles.

Packaging, Storage & Formulations

• Storage: Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.
• Buffer: 0.1 mg cell homogenate lyophilized in RIPA buffer made with double-knockout cell lines.
• Concentration: LYOPH
• Reconstitution Instructions: To use as WB negative control, spin down briefly and resuspend in 100 uL 1xSDS sample buffer (2% SDS, 60 mM Tris-HCl pH 6.8, 10% Glycerol, 0.02% Bromophenol blue, 60 mM beta-mercaptoethanol). Boil the lysate for 3 - 5 minutes before loading it onto gel.

Lysate Details for Array

• Type: Knockout 293T Cell
• Life Stage: Embryonic
• Tissue: Kidney

Notes

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Validation of antibody specificity is critical and verification of antibody performance against knockout samples is one way to guarantee that an antibody recognizes a specific target. Novus' KO cell lysate can be used as a negative control for western blots and to confirm the specificity of antibodies.

Alternate Names for FANCI Knockout 293T Cell Lysate

• Fanconi anemia group I protein
• Fanconi anemia, complementation group I
• KIAA1794FLJ10719
• Protein FACI

Background

FANCI is the gene underlying Fanconi anemia complementation group I, a syndrome characterized by a predisposition to cancer, congenital malformations, and pancytopenia. At the cellular level, FANCI is involved in the S and G2 phase DNA damage checkpoint and in the repair of DNA double-strand breaks and cross-links.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Bioinformatics

• Gene Symbol: FANCI