FANCC Antibody

Western Blot: FANCC Antibody [NBP1-18979] - Western Blot on cells overexpressing FANCC probed with 1. pre-immune serum and 2. anti-FANCC [NBP1-18979].

Western Blot: FANCC Antibody [NBP1-18979] - Western blot analysis of FANCC expression in 1) NTera2 and 2) Cos 7 whole cell lysates.

Product Discontinued

FANCC Antibody Summary

• Immunogen: N-terminal human FANCC protein [Swiss-Prot #Q00597].
• Localization: Nucleus. Cytoplasm. Note= The major form is nuclear. The minor form is cytoplasmic.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: FANCC
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:10-1:1000
  • Immunocytochemistry/ Immunofluorescence 1:50-1:100
  • Immunoprecipitation 1:10-1:500
  • Western Blot 1:500-1:1000
• Application Notes: This FANCC antibody is useful for Flow Cytometry, Immunocytochemistry/Immunofluorescence, Immunoprecipitation and Western blot, where a band is seen ~63 kDa.

Reactivity Notes

Human and primate.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Whole antisera
• Preservative: No Preservative
• Purity: Unpurified

Alternate Names for FANCC Antibody

• FA Complementation Group C
• FA3
• FACC
• FACCProtein FACC
• FACFanconi anemia group C protein
• FANCC
• Fanconi anemia, complementation group C
• FLJ14675

Background

FANCC (Fanconi anemia group C protein) is a DNA repair protein which implicates in post-replication DNA repair or a cell cycle checkpoint function. FANCC also involves in the interstrand DNA cross-link repair as well as the maintenance of chromosome stability/integrity. FANCC participates in the multisubunit FA complex (FANCA, FANCB, FANCC, FANCE, FANCF, FANCG, FANCL/PHF9, FANCM and HSP70) which is not found in FA patients. Upon IFNG induction, FANCC facilitate STAT1 activation via recruitement of STAT1 to IFNGR1. FANCC interacts with ZBTB32 (upon IFNG induction), CDK1, STAT1 etc and FANCC-EIF2AK2 interactions leads to augmented EIF2AK2 activation and cell death. FANCC is expressed ubiquitously and the major form is nuclear while the minor form is cytoplasmic. Defects in FANCC are the cause of Fanconi anemia complementation group C (FANCC), disorder affecting all bone marrow elements and resulting in anemia, leukopenia and thrombopenia.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: FANCC
• Entrez:
  • 2176(Human)
• Uniprot:
  • Q00597(Human)