FAM129B Antibody - BSA Free

Western Blot: FAM129B Antibody [NBP1-88784] - Analysis using Anti-FAM129B antibody NBP1-88784 (A) shows similar pattern to independent antibody NBP1-88783 (B).

Western Blot: FAM129B Antibody [NBP1-88784] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.

Immunocytochemistry/Immunofluorescence: FAM129B Antibody [NBP1-88784] - Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm, plasma membrane & cytosol.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

FAM129B Antibody - BSA Free Summary

• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: LIGNSLPGTTAKSGSAPILKCPTQFPLILWHPYARHYYFCMMTEAEQDKWQAVLQDCIRHCNNGIPEDSKVEGPAFTDAIRMYRQSKELYGTWEMLCGNE
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: NIBAN2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry Validated in publication (Cai L, Zhang S, Yang Z et al.)
  • Immunohistochemistry-Frozen Validated in publication (Cai L, Zhang S, Yang Z et al.)
  • Immunohistochemistry-Paraffin Validated in publication (Cai L, Zhang S, Yang Z et al.)
  • Western Blot 0.04-0.4 ug/ml
• Application Notes: ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
• Control Peptide:

  FAM129B Protein (NBP1-88784PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for FAM129B Antibody - BSA Free

• bA356B19.6
• C9orf88
• chromosome 9 open reading frame 88
• DKFZP434H0820
• family with sequence similarity 129, member B
• FLJ13518
• FLJ22151
• FLJ22298
• MEG-3
• MINERVA
• niban-like protein 1
• OC58
• Protein FAM129B

Background

Niban-like protein (NIBL) is a member of the niban family of proteins and contains a pleckstrin homology (PH) domain which is found in proteins involved in intracellular signaling and cytoskeletal organization. The function of niban and NIBL has not been fully elucidated.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for FAM129B Antibody (NBP1-88784)(2)

Publications using NBP1-88784

• Zhang S, Yang Z, Wei R et al. NIBAN2 promotes osteogenic differentiation and antagonizes osteoporosis by regulating RUNX2 alternative splicing Research Square 2023-01-04 (IHC, WB, IF, Mouse)
• Cai L, Zhang S, Yang Z et al. Identification of NIBAN2-regulated RUNX2 alternative splicing presents novel strategies antagonizing osteoporosis Research Square 2023-08-30 (IHC-Fr, WB, IHC-P, Mouse)

Bioinformatics

• Gene Symbol: NIBAN2