| Reactivity | HuSpecies Glossary |
| Applications | WB, ELISA |
| Clone | RFF-VIIIC/10 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | DyLight 755 |
| Immunogen | Affinity purified human Factor VIII |
| Specificity | This antibody recognises human Factor VIII, an essential blood coagulation factor. Whilst circulating in the blood, it is mostly stably complexed to von Willebrand factor. It is activated through cleavage at various sites, dissociates from the complex and interacts with Factor IXa, in the presence of calcium ions and phospholipids, to convert Factor X to the activated Factor Xa, which activates thrombin. Thrombin cleaves fibrinogen into fibrin, which polymerises and cross-links to form a blood clot. The activated Factor VIII is proteolytically inactivated and cleared from the bloodstream. Clone RFF-VIIIC/10 recognises an epitope within the non-functional, middle domain of full-length Factor VIII. |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | F8 |
| Purity | Protein G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
| Application Notes | Optimal dilution of this antibody should be experimentally determined. |
| Storage | Store at 4C in the dark. |
| Buffer | 50mM Sodium Borate |
| Preservative | 0.05% Sodium Azide |
| Purity | Protein G purified |
![Factor VIII Antibody (RFF-VIIIC/10) [DyLight 755]](/sites/all/modules/enterprise-tech/et_datasheets/images/novus_guarantee.png "Factor VIII Antibody (RFF-VIIIC/10) [DyLight 755]")
Secondary Antibodies |
Isotype Controls |
| Alexa Fluor 350 | NBP1-41021AF350 | |
| Alexa Fluor 405 | NBP1-41021AF405 | |
| Alexa Fluor 488 | NBP1-41021AF488 | |
| Alexa Fluor 532 | NBP1-41021AF532 | |
| Alexa Fluor 594 | NBP1-41021AF594 | |
| Alexa Fluor 647 | NBP1-41021AF647 | |
| Alexa Fluor 700 | NBP1-41021AF700 | |
| Alexa Fluor 750 | NBP1-41021AF750 | |
| Biotin | NBP1-41021B | |
| DyLight 350 | NBP1-41021UV | |
| DyLight 405 | NBP1-41021V | |
| DyLight 488 | NBP1-41021G | |
| DyLight 550 | NBP1-41021R | |
| DyLight 594 | NBP1-41021DL594 | |
| DyLight 650 | NBP1-41021C | |
| DyLight 680 | NBP1-41021FR | |
| DyLight 755 | NBP1-41021IR | |
| FITC | NBP1-41021F | |
| HRP | NBP1-41021H |
Diseases for Factor VIII Antibody (NBP1-41021IR)Discover more about diseases related to Factor VIII Antibody (NBP1-41021IR).
| Pathways for Factor VIII Antibody (NBP1-41021IR)View related products by pathway.
|
PTMs for Factor VIII Antibody (NBP1-41021IR)Learn more about PTMs related to Factor VIII Antibody (NBP1-41021IR).
|
|
Factor VIII - a key factor in the clotting process Hemostasis, or blood clotting, follows tissue injury and involves the deployment of essential plasma procoagulants (such as prothrombin, and Factors X, IX, V, and VIII) that trigger the blood coagulation cascade. This cascade leads to the formation... Read full blog post. |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
![Western Blot: Factor VIII Antibody [NB100-91761] - Detection of Factor VIII protein in extracts from human umbilical vein endothelial cells (HUVECs).](http://images.novusbio.com/images2/Factor-VIII-Antibody-Western-Blot-NB100-91761-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: Factor VIII Antibody [NB100-91761] - Factor VIII antibody was tested 1:50 in HeLa cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red). Image objective 40X.](http://images.novusbio.com/images2/Factor-VIII-Antibody-Immunocytochemistry-Immunofluorescence-NB100-91761-img0005.jpg)
![Immunohistochemistry-Paraffin: Factor VIII Antibody [NB100-91761] - Staining of Factor VIII in a formalin fixed and paraffin embedded tissue section of mouse bladder. The primary antibody was used at 1:100 dilution and the detection was performed using DAB with hematoxylin counterstaining.](http://images.novusbio.com/images2/Factor-VIII-Antibody-Immunohistochemistry-Paraffin-NB100-91761-img0007.jpg)
![Dual RNAscope ISH-IHC: Von Willebrand Factor Antibody [NB600-586] - Formalin-fixed paraffin-embedded tissue sections of human metastatic tonsil were probed for vWF mRNA (ACD RNAScope probe, catalog # 5860461; Fast Red chromogen, ACD catalog # 322500). Adjacent tissue section was processed for immunohistochemistry using rabbit polyclonal (Novus catalog # NB600-586) at 1:500 dilution for 1 hour at room temperature followed by incubation with the anti-rabbit IgG VisUCyte HRP Polymer Antibody (Catalog # VC003) and DAB chromogen (yellow-brown). Tissue was counterstained with hematoxylin (blue).](http://images.novusbio.com/images2/Von-Willebrand-Factor-Antibody-Dual-RNAscope-ISH-IHC-NB600-586-img0007.jpg)
![Immunohistochemistry: Von Willebrand Factor Antibody [NB600-586] - Formalin fixed paraffin embedded human tonsil stained with Factor VIII antibody.](http://images.novusbio.com/images2/Von-Willebrand-Factor-Antibody-Immunohistochemistry-NB600-586-img0001.jpg)
![Immunohistochemistry-Paraffin: Von Willebrand Factor Antibody [NB600-586] - Staining of formalin-fixed, paraffin-embedded Human Kidney sections with 1:2,000 Rabbit Anti-von Willebrand Factor.](http://images.novusbio.com/images2/Von-Willebrand-Factor-Antibody-Immunohistochemistry-Paraffin-NB600-586-img0005.jpg)
![Western Blot: Coagulation Factor II/Thrombin Antibody [NBP1-58268] - 721_B tissue lysate at a concentration of 1ug/ml.](http://images.novusbio.com/images2/Coagulation-Factor-II-Thrombin-Antibody-Western-Blot-NBP1-58268-img0007.jpg)
![Immunohistochemistry: Coagulation Factor II/Thrombin Antibody [NBP1-58268] - Analysis of human liver after heat-induced Antigen retrieval. Antibody concentration 5 ug/ml.](http://images.novusbio.com/images2/Coagulation-Factor-II-Thrombin-Antibody-Immunohistochemistry-NBP1-58268-img0008.jpg)
![Immunohistochemistry-Paraffin: Coagulation Factor II/Thrombin Antibody [NBP1-58268] - Human Placenta Tissue](http://images.novusbio.com/images2/Coagulation-Factor-II-Thrombin-Antibody-Immunohistochemistry-Paraffin-NBP1-58268-img0005.jpg)
![Western Blot: Coagulation Factor X Antibody [NBP1-33320] - HepG2 whole cell extract and conditioned medium (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Factor X antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/images2/Coagulation-Factor-X-Antibody-Western-Blot-NBP1-33320-img0013.jpg)
![Immunocytochemistry/Immunofluorescence: Coagulation Factor X Antibody [NBP1-33320] - Methanol-fixed HeLa, using Factor X antibody at 1:200 dilution.](http://images.novusbio.com/images2/Coagulation-Factor-X-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-33320-img0008.jpg)
![Immunohistochemistry-Paraffin: Coagulation Factor X Antibody [NBP1-33320] - NCI-N87 xenograft, using Factor X antibody at 1:100 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/images2/Coagulation-Factor-X-Antibody-Immunohistochemistry-Paraffin-NBP1-33320-img0009.jpg)
![Western Blot: Albumin Antibody [NB600-41532] - Analysis using the HRP conjugate of NB600-41532. Detection of Albumin in whole mouse liver extracts. Image courtesy of anonymous customer product review.](http://images.novusbio.com/images2/Albumin-Antibody-Western-Blot-NB600-41532-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: Albumin Antibody [NB600-41532] - NLRP12 downregulates multiple JNK activating pathways in hepatocytes. For checking the purity of primary hepatocytes, hepatocytes isolated from healthy WT and Nlrp12-/- mouse livers were immunostained with antibody for hepatocyte-specific marker Albumin and counterstained with DAPI. Image collected and cropped by CiteAb from the following publication (https://elifesciences.org/articles/40396), licensed under a CC-BY licence.](http://images.novusbio.com/images2/Albumin-Antibody-Immunocytochemistry-Immunofluorescence-NB600-41532-img0003.jpg)
![SDS-Page: Recombinant Human PRH2 Protein [H00005555-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.](http://images.novusbio.com/images/qc_test-H00005555-P01-1.jpg)
![SDS-Page: Human Thymosin beta 4 Protein [NBP2-35087]](http://images.novusbio.com/images2/Human-Thymosin-beta-4-Protein-SDS-Page-NBP2-35087-img0001.jpg)
![Western Blot: TSTA3 Antibody [NBP1-59130] - Hela, Antibody Dilution: 1.0 ug/ml TSTA3 is strongly supported by BioGPS gene expression data to be expressed in HeLa.](http://images.novusbio.com/images2/TSTA3 Antibody-Western Blot-NBP1-59130-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: TSTA3 Antibody [NBP1-59130] - Antibody Formalin Fixed Paraffin Embedded Tissue: Human Adult Liver Observed Staining: Cytoplasm in hepatocytes, strong signal, low tissue distribution P](http://images.novusbio.com/images2/TSTA3-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-59130-img0003.jpg)
![Western Blot: TSTA3 Antibody [NBP1-59130] - MCF-7 whole cell lysates, concentration 0.2-1 ug/ml.](http://images.novusbio.com/images2/TSTA3-Antibody-Western-Blot-NBP1-59130-img0002.jpg)
![ELISA: Human Factor IX ELISA Kit (Colorimetric) [NBP2-60518] - Standard curve.](http://images.novusbio.com/images2/Human-Factor-IX-ELISA-Kit-(Colorimetric)-ELISA-NBP2-60518-img0001.jpg)
![ELISA: Human Factor IX ELISA Kit (Colorimetric) [NBP2-60518] - Standard curve.](http://images.novusbio.com/images2/Human-Factor-IX-ELISA-Kit-(Colorimetric)-ELISA-NBP2-60518-img0002.jpg)
![Western Blot: hnRNP C1 + C2 Antibody (SN0652) [NBP2-67656] - Analysis of hnRNP C1+C2 on different lysates using anti-hnRNP C1+C2 antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: MCF-7 Lane 3: HepG2](http://images.novusbio.com/images2/hnRNP-C1-+-C2-Antibody-(SN0652)-Western-Blot-NBP2-67656-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: hnRNP C1 + C2 Antibody (SN0652) [NBP2-67656] - Staining hnRNP C1+C2 in B16-F1 (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/images2/hnRNP C1 + C2 Antibody (SN0652)-Immunocytochemistry-Immunofluorescence-NBP2-67656-img0010.jpg)
![Immunohistochemistry-Paraffin: hnRNP C1 + C2 Antibody (SN0652) [NBP2-67656] - Analysis of paraffin-embedded mouse skin tissue using anti-hnRNP C1+C2 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/images2/hnRNP-C1-+-C2-Antibody-(SN0652)-Immunohistochemistry-Paraffin-NBP2-67656-img0008.jpg)
![Western Blot: GPHA2 Antibody [NBP3-04883] - Analysis of extracts of rat brain, using GPHA2 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit.](http://images.novusbio.com/images2/GPHA2 Antibody-Western Blot-NBP3-04883-img0001.jpg)
![Western Blot: alpha-Sarcoglycan Antibody (JA51-81) [NBP2-67150] - Analysis of alpha sarcoglycan on rat heart tissue lysates using anti-alpha sarcoglycan antibody at 1:500 dilution.](http://images.novusbio.com/images2/alpha-Sarcoglycan Antibody (JA51-81)-Western Blot-NBP2-67150-img0003.jpg)
![Immunohistochemistry-Frozen: alpha-Sarcoglycan Antibody (JA51-81) [NBP2-67150] - Human skeletal muscle tissue. IHC-Fr image submitted by a verified customer review.](http://images.novusbio.com/images2/alpha-Sarcoglycan-Antibody-(JA51-81)-Immunohistochemistry-Frozen-NBP2-67150-img0005.jpg)
![Immunohistochemistry-Paraffin: alpha-Sarcoglycan Antibody (JA51-81) [NBP2-67150] - Analysis of paraffin-embedded mouse heart tissue using anti-alpha sarcoglycan antibody. Counter stained with hematoxylin.](http://images.novusbio.com/images2/alpha-Sarcoglycan-Antibody-(JA51-81)-Immunohistochemistry-Paraffin-NBP2-67150-img0001.jpg)
![Western Blot: ATP6V0A2 Antibody [NBP1-59069] - HeLa cells, concentration 3.3 ug/ml.](http://images.novusbio.com/images2/ATP6V0A2-Antibody-Western-Blot-NBP1-59069-img0002.jpg)
![Immunohistochemistry: ATP6V0A2 Antibody [NBP1-59069] - Human kidney lysate tissue at an antibody concentration of 5.0 ug/ml using anti-ATP6V0A2 antibody](http://images.novusbio.com/images2/ATP6V0A2-Antibody-Immunohistochemistry-NBP1-59069-img0008.jpg)
![Western Blot: ATP6V0A2 Antibody [NBP1-59069] - Hela tissue lysate at a concentration of 1ug/ml.](http://images.novusbio.com/images2/ATP6V0A2-Antibody-Western-Blot-NBP1-59069-img0001.jpg)
![ELISA: Human Factor V ELISA Kit (Colorimetric) [NBP2-82505] - Standard Curve Reference](http://images.novusbio.com/images2/Human Factor V ELISA Kit (Colorimetric)-ELISA-NBP2-82505-img0001.jpg)
![ELISA: Human Complement Component C2 ELISA Kit (Colorimetric) [NBP2-60548] - Standard curve.](http://images.novusbio.com/images2/Human-Complement-Component-C2-ELISA-Kit-(Colorimetric)-ELISA-NBP2-60548-img0001.jpg)
![Western Blot: Myoglobin Antibody (SC06-38) [NBP2-67599] - Analysis of Myoglobin on human skeletal muscle lysates using anti-Myoglobin antibody at 1/1,000 dilution.](http://images.novusbio.com/images2/Myoglobin Antibody (SC06-38)-Western Blot-NBP2-67599-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: Myoglobin Antibody (SC06-38) [NBP2-67599] - Staining Myoglobin in C2C12 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/images2/Myoglobin-Antibody-(SC06-38)-Immunocytochemistry-Immunofluorescence-NBP2-67599-img0001.jpg)
![Immunohistochemistry-Paraffin: Myoglobin Antibody (SC06-38) [NBP2-67599] - Analysis of paraffin-embedded mouse skeletal muscle tissue using anti-Myoglobin antibody. Counter stained with hematoxylin.](http://images.novusbio.com/images2/Myoglobin-Antibody-(SC06-38)-Immunohistochemistry-Paraffin-NBP2-67599-img0003.jpg)