Exportin-5 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: Exportin-5 Antibody [NBP3-38333] - Immunofluorescence analysis of MCF7 cells using Exportin-5(XPO5) Rabbit pAb.Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution.

Western Blot: Exportin-5 Antibody [NBP3-38333] - Western blot analysis of various lysates, using Exportin-5(XPO5) Rabbit pAb at 1:10000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.

Immunocytochemistry/ Immunofluorescence: Exportin-5 Antibody [NBP3-38333] - Immunofluorescence analysis of PC-12 cells using Exportin-5(XPO5) Rabbit pAb at dilution of 1:500 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Immunoprecipitation: Exportin-5 Antibody [NBP3-38333] - Immunoprecipitation analysis of 200 ug extracts of HeLa cells using Exportin-5(Exportin 5 (XPO5)) antibody. Western blot was performed from the immunoprecipitate using Exportin 5 (Exportin 5 (XPO5)) antibody at a dilution of 1:1000.

Immunocytochemistry/ Immunofluorescence: Exportin-5 Antibody [NBP3-38333] - Immunofluorescence analysis of MCF7 cells using Exportin-5 Rabbit pAb at dilution of 1:500 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA, ICC/IF, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

Exportin-5 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit Exportin-5 Antibody - BSA Free (NBP3-38333) is a polyclonal antibody validated for use in WB, ELISA, ICC/IF and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 925-1204 of human Exportin-5 (Exportin-5 (XPO5)) (NP_065801.1).
  
  Sequence:
  MRLSQKWQVINQRSLLCGEDEAADENPESQEMLEEQLVRMLTREVMDLITVCCVSKKGADHSSAPPADGDDEEMMATEVTPSAMAELTDLGKCLMKHEDVCTALLITAFNSLAWKDTLSCQRTTSQLCWPLLKQVLSGTLLADAVTWLFTSVLKGLQMHGQHDGCMASLVHLAFQIYEALRPRYLEIRAVMEQIPEIQKDSLDQFDCKLLNPSLQKVADKRRKDQFKRLIAGCIGKPLGEQFRKEVHIKNLPSLFKKTKPMLETEVLDNDGGGLATIFEP
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: XPO5
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence 1:100 - 1:500
  • Immunoprecipitation 0.5ug - 4ug antibody for 200ug - 400ug extracts of whole cells
  • Western Blot 1:2000 - 1:10000
• Theoretical MW: 136 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 50% glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for Exportin-5 Antibody - BSA Free

• Exp5
• exportin 5
• exportin-5
• FLJ14239
• FLJ32057
• FLJ45606
• KIAA1291exp5
• Ran-binding protein 21
• RANBP21

Background

Exportin-5 belongs to a large family of karyopherins (see MIM 602738) that mediate the transport of proteins and other cargo between the nuclear and cytoplasmic compartments.[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: XPO5