![Immunohistochemistry-Paraffin: Ets-1 Antibody (ETS1/1801) - Azide and BSA Free [NBP2-54514] - Formalin-fixed, paraffin-embedded human Spleen stained with ETS1 Monoclonal Antibody (ETS1/1282).](http://images.novusbio.com/fullsize2/Ets-1-Antibody-(ETS1-1801)---Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-54514-img0001.jpg "Immunohistochemistry-Paraffin: Ets-1 Antibody (ETS1/1801) - Azide and BSA Free [NBP2-54514] - Formalin-fixed, paraffin-embedded human Spleen stained with ETS1 Monoclonal Antibody (ETS1/1282).")
| Reactivity | Hu, MuSpecies Glossary |
| Applications | WB, Flow, ICC/IF, IHC, IHC-P, PA, CyTOF-ready |
| Clone | ETS1/1801 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Concentration | 1 mg/ml |
| Immunogen | Recombinant human ETS1 fragment (aa137-230) (exact sequence is proprietary) |
| Localization | Nuclear |
| Marker | Marker of Tumor Metastasis |
| Isotype | IgG2b Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | ETS1 |
| Purity | Protein A or G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | IHC-P: Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA pH 9.0 for 10-20 min followed by cooling at RT for 20 minutes |
| Theoretical MW | 54 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at -20 to -80C. Avoid freeze-thaw cycles. |
| Buffer | 10 mM PBS |
| Preservative | No Preservative |
| Concentration | 1 mg/ml |
| Purity | Protein A or G purified |
Secondary Antibodies |
Isotype Controls |
| Alexa Fluor 350 | NBP2-54514AF350 | |
| Alexa Fluor 405 | NBP2-54514AF405 | |
| Alexa Fluor 488 | NBP2-54514AF488 | |
| Alexa Fluor 532 | NBP2-54514AF532 | |
| Alexa Fluor 594 | NBP2-54514AF594 | |
| Alexa Fluor 647 | NBP2-54514AF647 | |
| Alexa Fluor 700 | NBP2-54514AF700 | |
| Alexa Fluor 750 | NBP2-54514AF750 | |
| Allophycocyanin | NBP2-54514APC | |
| Biotin | NBP2-54514B | |
| DyLight 350 | NBP2-54514UV | |
| DyLight 405 | NBP2-54514V | |
| DyLight 488 | NBP2-54514G | |
| DyLight 550 | NBP2-54514R | |
| DyLight 594 | NBP2-54514DL594 | |
| DyLight 650 | NBP2-54514C | |
| DyLight 680 | NBP2-54514FR | |
| DyLight 755 | NBP2-54514IR | |
| FITC | NBP2-54514F | |
| HRP | NBP2-54514H | |
| Janelia Fluor 549 | NBP2-54514JF549 | |
| Janelia Fluor 646 | NBP2-54514JF646 | |
| PE | NBP2-54514PE | |
| PerCP | NBP2-54514PCP |
Diseases for Ets-1 Antibody (NBP2-54514)Discover more about diseases related to Ets-1 Antibody (NBP2-54514).
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PTMs for Ets-1 Antibody (NBP2-54514)Learn more about PTMs related to Ets-1 Antibody (NBP2-54514).
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HIF-3 alpha: a versatile target with hypoxia dependent and independent functions By: Subhash GangarHIF-3 alpha (hypoxia-inducible factor 3-alpha/ HIF3A) represents an isoform of HIF-alpha subunits which heterodimerize with stable beta subunit (HIF-beta) for the regulation of HIF target genes through binding to hypoxia respon... Read full blog post. |
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| Gene Symbol | ETS1 |
![Protein Array: Ets-1 Antibody (ETS1/1801) - Azide and BSA Free [NBP2-54514] - Analysis of Protein Array containing more than 19,000 full-length human proteins using Ets-1 Antibody (ETS1/1801) Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (Monoclonal Antibody) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SDs) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SDs) between the Z-score. S-score therefore represents the relative target specificity of a Monoclonal Antibody to its intended target. A Monoclonal Antibody is considered to specific to its intended target, if the Monoclonal Antibody has an S-score of at least 2.5.](http://images.novusbio.com/fullsize2/Ets-1-Antibody-(ETS1-1801)---Azide-and-BSA-Free-Protein-Array-NBP2-54514-img0002.jpg "Protein Array: Ets-1 Antibody (ETS1/1801) - Azide and BSA Free [NBP2-54514] - Analysis of Protein Array containing more than 19,000 full-length human proteins using Ets-1 Antibody (ETS1/1801) Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (Monoclonal Antibody) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SDs) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SDs) between the Z-score. S-score therefore represents the relative target specificity of a Monoclonal Antibody to its intended target. A Monoclonal Antibody is considered to specific to its intended target, if the Monoclonal Antibody has an S-score of at least 2.5.")
![Western Blot: c-jun Antibody (5B1) [NBP2-37576] - Analysis using c-Jun mouse mAb against NIH/3T3 (1) and Cos7 (2) cell lysate.](http://images.novusbio.com/images2/c-jun-Antibody-(5B1)-Western-Blot-NBP2-37576-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: c-jun Antibody (5B1) [NBP2-37576] - Analysis of PC-2 cells using c-Jun mouse mAb (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.](http://images.novusbio.com/images2/c-jun-Antibody-(5B1)-Immunocytochemistry-Immunofluorescence-NBP2-37576-img0004.jpg)
![Immunohistochemistry-Paraffin: c-jun Antibody (5B1) [NBP2-37576] - Analysis of human rectum cancer tissues using c-Jun mouse mAb with DAB staining.](http://images.novusbio.com/images2/c-jun-Antibody-(5B1)-Immunohistochemistry-NBP2-37576-img0007.jpg)
![Western Blot: ERK1 Antibody (1E5) [NBP2-22203] - Western blot analysis of whole cell lysates from (1) MCF7 (2) NIH3T3 cell lines using ERK1 antibody (clone 1E5) at 1:1000 dilution. The signal was developed using HRP labeled goat-anti Mouse secondary antibody with ECL based detection.](http://images.novusbio.com/images2/ERK1-Antibody-(1E5)-Western-Blot-NBP2-22203-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: ERK1 Antibody (1E5) [NBP2-22203] - Analysis of NIH/3T3 cells using ERK1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.](http://images.novusbio.com/images2/ERK1-Antibody-(1E5)-Immunocytochemistry-Immunofluorescence-NBP2-22203-img0002.jpg)
![Immunohistochemistry-Paraffin: ERK1 Antibody (1E5) [NBP2-22203] - Immunohistochemical analysis of paraffin-embedded breast cancer tissues using ERK1 mouse mAb with DAB staining.](http://images.novusbio.com/images2/ERK1-Antibody-(1E5)-Immunohistochemistry-Paraffin-NBP2-22203-img0004.jpg)
![Knockout Validated: c-Fos Antibody (2H2) [NBP2-50037] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and c-Fox knockout (KO) HeLa cell line. PVDF membrane was probed with 1:1000 of Mouse Anti-Human c-Fox Monoclonal Antibody (Catalog # NBP2-50037) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog #HAF018). Specific band was detected for c-Fox at approximately 52 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.](http://images.novusbio.com/images2/c-Fos-Antibody-(2H2)-Knockout-Validated-NBP2-50037-img0005.jpg)
![Western Blot: c-Fos Antibody (2H2) [NBP2-50037] - Analysis of cell lysates using mouse c-Fos mAb, dilution 1:1,000 (Green), and rabbit GAPDH pAb, dilution 1:20,000 (Red) used as a loading control. [1] protein standard (red), [2] HeLa cells in serum free media. [3] HeLa cells stimulated with 20% fetal bovine serum for 2hrs after 36hrs in serum free media. [4] rat cortical neurons. [5] rat cortical neurons treated with membrane depolarization buffer for 5hrs. Multiple bands at 50-65kDa in stimulated or treated cell lysates correspond to different forms of the c-Fos proten. The single band at 37 kDa represents GAPDH protein.](http://images.novusbio.com/images2/c-Fos-Antibody-(2H2)-Western-Blot-NBP2-50037-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: c-Fos Antibody (2H2) [NBP2-50037] - Section of rat hippocampus stained with mouse monoclonal antibody to c-FOS NBP2-50037 in red and counterstained with rabbit polyclonal antibody to FOX3/NeuN. DAPI reveals nuclei of neurons and glia in blue. The hippocampal neurons stain green for FOX3/NeuN and a few also are expressing c-FOS, and so appear orange. These cells were spontaneously active at the time the animal was sacrificed.](http://images.novusbio.com/images2/c-Fos-Antibody-(2H2)-Immunocytochemistry-Immunofluorescence-NBP2-50037-img0006.jpg)
![Western Blot: ETS2 Antibody (OTI2A3) - Azide and BSA Free [NBP2-70694] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ETS2.](http://images.novusbio.com/images2/ETS2-Antibody-(OTI2A3)---Azide-and-BSA-Free-Western-Blot-NBP2-70694-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: ETS2 Antibody (OTI2A3) - Azide and BSA Free [NBP2-70694] - Analysis of COS7 cells transiently transfected by pCMV6-ENTRY ETS2.](http://images.novusbio.com/images2/ETS2 Antibody (OTI2A3) - Azide and BSA Free-Immunocytochemistry-Immunofluorescence-NBP2-70694-img0011.jpg)
![Immunohistochemistry: ETS2 Antibody (OTI2A3) - Azide and BSA Free [NBP2-70694] - Analysis of Human prostate tissue.](http://images.novusbio.com/images2/ETS2-Antibody-(OTI2A3)---Azide-and-BSA-Free-Immunohistochemistry-NBP2-70694-img0010.jpg)
![Western Blot: SP1 Antibody [NB600-233] - Samples: Whole cell lysate (50 ug) from HeLa, 293T, and mouse Renca cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-SP1 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/images2/SP1-Antibody-Western-Blot-NB600-233-img0007.jpg)
![Immunohistochemistry-Paraffin: SP1 Antibody [NB600-233] - Mouse renal cell carcinoma. Antibody: Affinity purified rabbit anti-SP1 used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB](http://images.novusbio.com/images2/SP1-Antibody-Immunohistochemistry-Paraffin-NB600-233-img0009.jpg)
![Chromatin Immunoprecipitation: SP1 Antibody [NB600-233] - Chromatin from acute myeloid leukemia cell line was immunoprecipitated with anti-Sp1 antibody and analyzed by DNA sequencing. The figure illustrates the peak distribution of Sp1 binding within a 250 Kb region of chromosome 6 asdetected using anti-Sp1.](http://images.novusbio.com/images2/SP1-Antibody-Chromatin-Immunoprecipitation-NB600-233-img0014.jpg)
![Western Blot: DAND5 Antibody (2H3) [NBP2-01223] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DAND5 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DAND5.](http://images.novusbio.com/images/DAND5 Antibody (2H3)-Western Blot-NBP2-01223-img0002.jpg)
![Immunohistochemistry-Paraffin: DAND5 Antibody (2H3) [NBP2-01223] Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-DAND5 mouse monoclonal antibody.](http://images.novusbio.com/images/DAND5 Antibody (2H3)-Immunohistochemistry-Paraffin-NBP2-01223-img0005.jpg)
![Flow Cytometry: DAND5 Antibody (2H3) [NBP2-01223] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-DAND5 antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/images/DAND5 Antibody (2H3)-Flow Cytometry-NBP2-01223-img0001.jpg)
![Western Blot: FosB/G0S3 Antibody (83B1138) [NB100-56530] - Analysis of FosB in HeLa cell lysate where bands are observed at ~38 kDa representing FosB and at ~35 kDa representing delta FosB.](http://images.novusbio.com/images2/FosB-G0S3-Antibody-(83B1138)-Western-Blot-NB100-56530-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: JunD [p Ser255] Antibody (JJ08-21) [NBP2-67861] - Staining Phospho-JunD(S255) in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/images2/JunD-[p-Ser255]-Antibody-(JJ08-21)-Immunocytochemistry-Immunofluorescence-NBP2-67861-img0003.jpg)
![Immunohistochemistry-Paraffin: JunD [p Ser255] Antibody (JJ08-21) [NBP2-67861] - Analysis of paraffin-embedded mouse liver tissue using anti-Phospho-JunD(S255) antibody. Counter stained with hematoxylin.](http://images.novusbio.com/images2/JunD-[p-Ser255]-Antibody-(JJ08-21)-Immunohistochemistry-Paraffin-NBP2-67861-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: JunD [p Ser255] Antibody (JJ08-21) [NBP2-67861] - Staining Phospho-JunD(S255) in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/images2/JunD-[p-Ser255]-Antibody-(JJ08-21)-Immunocytochemistry-Immunofluorescence-NBP2-67861-img0001.jpg)
![Western Blot: JunB/AP-1 Antibody [NBP1-89544] - Analysis in human cell lines MCF-7 and HEK293 using anti-JUNB antibody. Corresponding JUNB RNA-seq data are presented for the same cell lines. Loading control: anti-PFN1.](http://images.novusbio.com/images2/JunB-AP-1 Antibody-Western Blot-NBP1-89544-img0018.jpg)
![Immunocytochemistry/Immunofluorescence: JunB/AP-1 Antibody [NBP1-89544] - Staining of human cell line A-431 shows localization to nucleoplasm. Antibody staining is shown in green.](http://images.novusbio.com/images2/JunB-AP-1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-89544-img0011.jpg)
![Immunohistochemistry-Paraffin: JunB/AP-1 Antibody [NBP1-89544] - Staining in human fallopian tube and skeletal muscle tissues. Corresponding JUNB RNA-seq data are presented for the same tissues.](http://images.novusbio.com/images2/JunB-AP-1-Antibody-Immunohistochemistry-Paraffin-NBP1-89544-img0016.jpg)
![Immunocytochemistry/Immunofluorescence: DDX6 Antibody [NB200-191] - In normal condition, cells were fixed and stained for DDX6, that usually punctuate in process bodies (PBs). Primary Antibody rabbit-anti-DDX6 diluted 1:1000. This image was submitted via customer Review.](http://images.novusbio.com/images2/DDX6-Antibody-Immunocytochemistry-Immunofluorescence-NB200-191-img0008.jpg)
![Immunohistochemistry-Paraffin: DDX6 Antibody [NB200-191] - Section of human colon carcinoma (left) and mouse renal cell carcinoma(right). Antibody: Affinity purified rabbit anti-DDX6 used at a dilution of 1:200 (1ug/ml). Detection: DAB.](http://images.novusbio.com/images2/DDX6-Antibody-Immunohistochemistry-Paraffin-NB200-191-img0013.jpg)
![Immunoprecipitation: DDX6 Antibody [NB200-191] - Detection of human DDX6 by Western Blot and Immunoprecipitation. Samples: Whole cell lysate (15 and 50 ug) from HeLa cells. Antibodies: Affinity purified rabbit anti-DDX6 antibody NB200-191 used at 0.04 ug/ml for WB (A and B) and at 3 ug/mg lysate for IP. DDX6 was also immunoprecipitated using rabbit anti-DDX6 antibody NB200-192, but not with 'DDX6 Ab', which is to another epitope from DDX6. Normal rabbit IgG was used as a negative control. Detection: Chemiluminescence with an exposure time of 1 second (A & B).](http://images.novusbio.com/images2/DDX6-Antibody-Immunoprecipitation-NB200-191-img0012.jpg)
![Western Blot: ERG Antibody (SP06-04) [NBP2-67375] - Analysis of ERG on Jurkat cell lysates using anti-ERG antibody at 1/1,000 dilution.](http://images.novusbio.com/images2/ERG-Antibody-(SP06-04)-Western-Blot-NBP2-67375-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: ERG Antibody (SP06-04) [NBP2-67375] - Staining ERG in CRC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/images2/ERG-Antibody-(SP06-04)-Immunocytochemistry-Immunofluorescence-NBP2-67375-img0005.jpg)
![Immunohistochemistry-Paraffin: ERG Antibody (SP06-04) [NBP2-67375] - Analysis of paraffin-embedded mouse heart tissue using anti-ERG antibody. Counter stained with hematoxylin.](http://images.novusbio.com/images2/ERG-Antibody-(SP06-04)-Immunohistochemistry-Paraffin-NBP2-67375-img0003.jpg)
