ETFDH Antibody

Western Blot: ETFDH Antibody [NBP1-52059] - NBP1-52059 (1ug/ml) staining of Human Kidney lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

• Reactivity: Hu, Mu, Rt, Bv, Ca, Rb
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

ETFDH Antibody Summary

• Description: Novus Biologicals Goat ETFDH Antibody (NBP1-52059) is a polyclonal antibody validated for use in WB and ELISA. Anti-ETFDH Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence C-EHDQPAHLTLRD corresponding to internal region according to NP_004444.2.
• Epitope: C-EHDQPAHLTLRD
• Predicted Species: Canine (100%), Bovine (100%), Rabbit (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: ETFDH
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:2000
  • Western Blot 1 - 3 ug/ml
• Application Notes: WB: Approx. 70 kDa band observed in human, mouse and rat heart lysates (calculated MW of 68.5 kDa band according to NP_004444.2).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for ETFDH Antibody

• electron-transferring-flavoprotein dehydrogenaseEC 1.5.5.1
• ETFQO
• ETF-QO
• MADD
• mitochondrial

Background

Electron-transferring-flavoprotein dehydrogenase in the inner mitochondrial membrane accepts electrons from electron-transfer flavoprotein which is located in the mitochondrial matrix and reduces ubiquinone in the mitochondrial membrane. The protein is synthesized as a 67-kDa precursor which is targeted to mitochondria and processed in a single step to a 64-kDa mature form located in the mitochondrial membrane. Deficiency in electron-transferring-flavoprotein dehydrogenase have been demonstrated in some patients with type II glutaricacidemia. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ETFDH Antibody (NBP1-52059)(1)

Publication using NBP1-52059

• Illig T, Gieger C, Zhai G et al. A genome-wide perspective of genetic variation in human metabolism. Nat Genet 2010-02-01 [PMID: 20037589]

Bioinformatics

• Gene Symbol: ETFDH
• Entrez:
  • 2110(Human)
  • 66841(Mouse)
  • 295143(Rat)
• Uniprot:
  • NP_004444.2(Human)