Human ESAM ELISA Kit (Colorimetric) Summary
Description |
Assay Length: 4.5 hours |
Standard Curve Range |
0.312 - 20 ng/mL (example only; lot dependent) |
Sensitivity |
0.117 ng/mL (example only; lot dependent) |
Assay Type |
Sandwich ELISA |
Inter-Assay |
%CV < 12 (example only; lot dependent) |
Intra-Assay |
%CV < 10 (example only; lot dependent) |
Sample Volume |
100 uL |
Kit Type |
ELISA Kit (Colorimetric) |
Gene |
ESAM |
Applications/Dilutions
Packaging, Storage & Formulations
Storage |
Storage of components varies. See protocol for specific instructions. |
Kit Components
Components
|
- Detection Reagent A
- Detection Reagent B
- Diluent Buffer
- Instruction manual
- Plate sealer for 96 wells
- Pre-coated 96T strip plate
- Standard
- Stop Solution
- TMB Substrate
- Wash Buffer (30 x concentrate)
|
Alternate Names for Human ESAM ELISA Kit (Colorimetric)
Background
ESAM is a tight junction regulatory protein involved in cell adhesion and is expressed only on endothelial cells and activated platelets. It may mediate aggregation through a homophilic molecular interaction (By similarity). ESAM at endothelial tight junctions participates in the migration of neutrophils through the vessel walls and it may have a role in signaling mechanisms that trigger the opening of endothelial cell contacts. A correlation has been found between ESAM expression and lymph node metastasis.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are
guaranteed for 6 months from date of receipt.
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