ERR alpha/NR3B1 Antibody

Product Discontinued

ERR alpha/NR3B1 Antibody Summary

• Immunogen: Synthetic 15 amino acid peptide from internal region of human ESRRA.
• Localization: Nuclear
• Specificity: Human ESRRA. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
• Predicted Species: Primate (93%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ESRRA
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Chromatin Immunoprecipitation 1:10 - 1:500
  • Immunohistochemistry 8-24 ug/ml
  • Immunohistochemistry-Paraffin 8-24 ug/ml
  • Western Blot
• Application Notes: Use in Western blot reported in scientific literature (PMID: 23720198).

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Gorilla (100%), Marmoset (100%), Elephant (100%), Panda (100%), Gibbon (93%). Rat reactivity reported in scientific literature (PMID: 23720198).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.1% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for ERR alpha/NR3B1 Antibody

• ERR alpha
• ERR1
• ERR1Estrogen receptor-like 1
• ERRa
• ESRL1
• ESRL1ERR-alpha
• ESRRA
• estrogen-related receptor alphaERRalpha
• NR3B1
• NR3B1Nuclear receptor subfamily 3 group B member 1
• steroid hormone receptor ERR1

Background

Estrogen-related receptor alpha (ERR alpha), a NR3 Steroid Receptor, was isolated based on sequence similarity in its DNA-binding domain to estrogen receptor alpha (ER alpha). ERR alpha has been shown to regulate the promoters of lactoferrin, medium-chain acyl CoA dehydrogenase, osteopontin, and thyroid receptor alpha, and it may affect cellular energy balance and bone formation. ERR alpha binds as a monodimer to the extended half-site TNAAGGTCA and as a homodimer to the estrogen response element (ERE) and is a constitutive activator of the estrogen response element and the palindromic thyroid hormone response element (TRE(pal)) but not of the glucocorticoid response element (GRE). ERR alpha 1 is the major isoform expressed in human breast cancer cell lines. Recent studies have shown that Phe-329 is responsible for the constitutive activity of ERR alpha. ERR alpha is a potential biomarker for unfavorable clinical outcome and, possibly, hormonal insensitivity in breast tumors. ERR alpha status may be predictive of sensitivity to hormonal blockade therapy, and ERR alpha status may also be predictive of ErbB2-based therapy such as Herceptin. Moreover, ERR alpha may be a candidate target for therapeutic development. ERRalpha null mice have altered regulation of genes involved in adipogenesis In mouse, ERR alpha is expressed in many adult and embryonic tissues (particularly at the onset of ossification) as well as in several osteoblast cell lines. ERR alpha expression has been documented in mouse in brain, spinal cord, pituitary gland, heart, intestine, bone, brown adipose tissue, heart, uterus, cervix, nerve, skeletal muscle, and vagina. ESTs have been isolated from human tissue libraries, including cancerous blood, brain, breast, cervix, colon, duodenum, eye, head/neck, kidney, liver, lung, ovary, pancreas, skeletal muscle, skin, stomach, and uterus, and normal adrenal, blood, brain, colon, embryo, eye, head/neck, heart, kidney, prostate, skeletal muscle, testis, and uterus.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ERR alpha/NR3B1 Antibody (NLS5402)(3)

Publications using NLS5402

• Bernatchez G, Giroux V, Lassalle T et al. The ERRalpha metabolic nuclear receptor controls growth of colon cancer cells. Carcinogenesis 2013-05-29 [PMID: 23720198] (WB, Rat, Human)
• Boudjadi S, Bernatchez G, Beaulieu JF, Carrier JC. Control of the Human Osteopontin Promoter by ERRalpha in Colorectal Cancer. Am J Pathol 2013-05-13 [PMID: 23680656] (IHC-P, WB, Human)
• Fritah A, Steel JH, Parker N, Nikolopoulou E, Christian M, Carling D, Parker MG. Absence of RIP140 Reveals a Pathway Regulating glut4-Dependent Glucose Uptake in Oxidative Skeletal Muscle through UCP1-Mediated Activation of AMPK. PLoS One;7(2):e32520. 2012-01-01 [PMID: 22389706]

Bioinformatics

• Gene Symbol: ESRRA
• Entrez:
  • 2101(Human)
• Uniprot:
  • P11474(Human)