ERR alpha/NR3B1 Antibody (SC57-07)

Western Blot: ERR alpha/NR3B1 Antibody (SC57-07) [NBP2-67556] - Analysis of Estrogen Related Receptor alpha on different lysates with Rabbit anti-Estrogen Related Receptor alpha antibody at 1/500 dilution. Lane 1: MCF-7 cell lysateLane 2: SK-Br-3 cell lysateLane 3: PC-3 cell lysateLysates/proteins at 10 ug/Lane. Predicted band size: 46 kDa Observed band size: 50 kDa, Exposure time: 2 minutes; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.

Immunohistochemistry-Paraffin: ERR alpha/NR3B1 Antibody (SC57-07) [NBP2-67556] - Flow cytometric analysis of Hela cells with Estrogen Related Receptor alpha antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.

Flow Cytometry: ERR alpha/NR3B1 Antibody (SC57-07) [NBP2-67556] - Analysis of Hela cells with Estrogen Related Receptor alpha antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the second

Immunohistochemistry-Paraffin: ERR alpha/NR3B1 Antibody (SC57-07) [NBP2-67556] - Analysis of paraffin-embedded mouse brain tissue using anti-Estrogen Related Receptor alpha antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Product Discontinued

ERR alpha/NR3B1 Antibody (SC57-07) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Synthetic peptide within Human ERR alpha/NR3B1 aa 1-49 / 423. (SwissProt: P11474 Human; SwissProt: O08580 Mouse; SwissProt: Q5QJV7 Rat)
• Localization: Nucleus.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: ESRRA
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Immunoprecipitation
  • Western Blot 1:1000-1:2000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for ERR alpha/NR3B1 Antibody (SC57-07)

• ERR alpha
• ERR1
• ERR1Estrogen receptor-like 1
• ERRa
• ESRL1
• ESRL1ERR-alpha
• ESRRA
• estrogen-related receptor alphaERRalpha
• NR3B1
• NR3B1Nuclear receptor subfamily 3 group B member 1
• steroid hormone receptor ERR1

Background

Estrogen-related receptor alpha (ERR alpha) is an orphan member of the superfamily of nuclear hormone receptors. ERR alpha was initially isolated based on its sequence homology to the estrogen receptor but is not activated by classic estrogens. Binding site selection experiments show that ERR alpha preferentially binds to an ERR alpha response element (ERRE) containing a single consensus half-site, TNAAGGTCA. Data demonstrate that ERR alpha can control the expression of MCAD through the NRRE-1 and thus may play an important role in regulating cellular energy balance in vivo (1). The estrogen-related receptors ERRalpha and ERRbeta (formerly ERR1 and ERR2) form a subgroup of the steroid/thyroid/retinoid receptor family. ERRalpha and ERRbeta are homologous to the estrogen receptor and bind similar DNA targets; however, they are unable to activate gene transcription in response to estrogens (2).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ESRRA