ERK3/MAPK6 Antibody (PSH03-39)

Western Blot: ERK3/MAPK6 Antibody (PSH03-39) [NBP3-32567] - Western blot analysis of ERK3/MAPK6 on different lysates with Rabbit anti-ERK3/MAPK6 antibody (NBP3-32567) at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: A431 cell lysate Lane 3: A549 cell lysate Lane 4: PC-3M cell lysate Lane 5: C2C12 cell lysate Lane 6: NIH/3T3 cell lysate Lane 7: C6 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 83 kDa Observed band size: 105 kDa Exposure time: 5 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32567) at 1/2,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.

Immunocytochemistry/ Immunofluorescence: ERK3/MAPK6 Antibody (PSH03-39) [NBP3-32567] - Immunocytochemistry analysis of 293T cells labeling ERK3/MAPK6 with Rabbit anti-ERK3/MAPK6 antibody (NBP3-32567) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-ERK3/MAPK6 antibody (NBP3-32567) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.

Flow Cytometry: ERK3/MAPK6 Antibody (PSH03-39) [NBP3-32567] - Flow cytometric analysis of 293T cells labeling ERK3/MAPK6. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32567, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

• Reactivity: Hu, Mu, Rt
• Applications: WB, Flow, ICC/IF
• Clone: PSH03-39
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

ERK3/MAPK6 Antibody (PSH03-39) Summary

• Description: Novus Biologicals Rabbit ERK3/MAPK6 Antibody (PSH03-39) (NBP3-32567) is a recombinant monoclonal antibody validated for use in WB, Flow and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Recombinant protein within human ERK3/MAPK6 aa 1-350 / 721. (Uniprot: Q16659)
• Localization: Cytoplasm, Nucleus.
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: MAPK6
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:1000
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Western Blot 1:2000
• Theoretical MW: 83 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH7.4), 0.1% BSA and 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for ERK3/MAPK6 Antibody (PSH03-39)

• DKFZp686F03189
• EC 2.7.11
• EC 2.7.11.24
• ERK3
• ERK-3
• ERK3p97-MAPK
• Extracellular signal-regulated kinase 3
• extracellular signal-regulated kinase, p97
• HsT17250
• MAP kinase 6
• MAP kinase isoform p97
• MAPK 6
• MAPK6
• mitogen-activated protein kinase 6
• p97MAPK
• PRKM6
• protein kinase, mitogen-activated 5
• protein kinase, mitogen-activated 6

Background

ERK3 (Extracellular signal-regulated kinase 3, MAPK6, p97 MAPK) belongs to the Ser/Thr protein kinase family and is a member of the mitogen-activated protein (MAP) kinase superfamily. Unlike the other members of the MAPK family, ERK3 is a mitogen-activated protein kinase homologue that has SEG motif instead of conserved TXY motif in the activation loop (1). Also, unlike ERK1 and ERK2, it is ubiquitously expressed and localized in the nucleus of proliferating cells (2). Highly unstable, ERK3 is constitutively degraded during ubiquitin-proteasome pathway of proliferating cells (3). ERK3 has been linked to phosphorylation MAP2 and as a possible regulator during cell differentiation (4).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: MAPK6