ERK4/MAPK4 Antibody (OTI2D7)

Images

 
Western Blot: ERK4/MAPK4 Antibody (OTI2D7) [NBP2-45743] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MAPK4 .
Immunohistochemistry: ERK4/MAPK4 Antibody (OTI2D7) [NBP2-45743] - Analysis of Human Kidney tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Western Blot: ERK4/MAPK4 Antibody (OTI2D7) [NBP2-45743] - Analysis of extracts (10ug) from 8 Human tissue (1: Testis; 2: Uterus; 3: Breast; 4: Brain; 5: Liver; 6: Ovary; 7: Thyroid gland; 8: Colon).
Immunohistochemistry: ERK4/MAPK4 Antibody (OTI2D7) [NBP2-45743] - Analysis of Adenocarcinoma of Human endometrium tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunohistochemistry: ERK4/MAPK4 Antibody (OTI2D7) [NBP2-45743] - Analysis of Human pancreas tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunohistochemistry: ERK4/MAPK4 Antibody (OTI2D7) [NBP2-45743] - Analysis of Adenocarcinoma of Human ovary tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunohistochemistry: ERK4/MAPK4 Antibody (OTI2D7) [NBP2-45743] - Analysis of Human tonsil tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunohistochemistry: ERK4/MAPK4 Antibody (OTI2D7) [NBP2-45743] - Analysis of Carcinoma of Human kidney tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunofluorescence: ERK4/MAPK4 Antibody (OTI2D7) [NBP2-45743] - Analysis of COS7 cells transiently transfected by pCMV6-ENTRY ERK4/MAPK4.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC, ICC/IF
Clone
OTI2D7
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

ERK4/MAPK4 Antibody (OTI2D7) Summary

Immunogen
Full length human recombinant protein of human MAPK4(NP_002738) produced in HEK293T cell.
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
MAPK4
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/Immunofluorescence 1:100
  • Immunofluorescence 1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin
  • Western Blot 1:4000
Theoretical MW
65.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for ERK4/MAPK4 Antibody (OTI2D7)

  • EC 2.7.11
  • EC 2.7.11.24
  • ERK3
  • Erk3-related
  • ERK4
  • ERK-4
  • Extracellular signal-regulated kinase 4
  • MAP kinase 4
  • MAP kinase isoform p63
  • MAPK 4
  • MAPK4
  • mitogen-activated protein kinase 4
  • p63Mapk
  • PRKM4
  • PRKM4p63-MAPK

Background

MAPK4 is a member of the Ser/Thr protein kinase family, and is most closely related to mitogen-activated protein kinases (MAP kinases). MAP kinases also known as extracellular signal-regulated kinases (ERKs), are activated through protein phosphorylation cascades and act as integration points for multiple biochemical signals. This kinase is localized in the nucleus, and has been reported to be activated in fibroblasts upon treatment with serum or phorbol esters.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP1-47842
Species: Ca, Hu, Pm, Mu, Rt
Applications: IHC, IHC-P, IP, WB
MAB3196
Species: Hu
Applications: IHC, WB
NBP2-22203
Species: Hu, Pm, Mu, Rt
Applications: ELISA, Flow, ICC/IF, IHC, IHC-P, WB
NBP1-87231
Species: Hu
Applications: ICC/IF, IHC, IHC-P
NBP2-13304
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
AF8691
Species: Hu, Mu, Rt
Applications: IHC, KO, Simple Western, WB
AF467
Species: Hu, Mu
Applications: CyTOF-ready, Flow, ICC, IHC, Simple Western, WB
AF2848
Species: Hu, Mu
Applications: ICC, WB
MAB17761
Species: Hu, Mu, Rt
Applications: ICC, WB
NBP2-37568
Species: Hu
Applications: ELISA, Flow, ICC/IF, IHC, IHC-P, WB
NBP2-00764
Species: Hu, Mu, Rt
Applications: Flow, IHC, IHC-P, WB
NBP1-81575
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
NBP1-68874
Species: Hu, Mu, Rt
Applications: PEP-ELISA, WB
H00010598-M01
Species: Hu, Mu, Rt
Applications: ELISA, ICC/IF, S-ELISA, WB
MAB8930
Species: Hu
Applications: ICC, Simple Western, WB
AF1347
Species: Hu, Mu, Rt
Applications: ICC, IHC, Simple Western, WB
MAB4540
Species: Hu, Mu, Rt
Applications: IHC, WB
NBP2-59376
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, IP, WB

Publications for ERK4/MAPK4 Antibody (NBP2-45743) (0)

There are no publications for ERK4/MAPK4 Antibody (NBP2-45743).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for ERK4/MAPK4 Antibody (NBP2-45743) (0)

There are no reviews for ERK4/MAPK4 Antibody (NBP2-45743). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for ERK4/MAPK4 Antibody (NBP2-45743) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional ERK4/MAPK4 Products

Bioinformatics Tool for ERK4/MAPK4 Antibody (NBP2-45743)

Discover related pathways, diseases and genes to ERK4/MAPK4 Antibody (NBP2-45743). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for ERK4/MAPK4 Antibody (NBP2-45743)

Discover more about diseases related to ERK4/MAPK4 Antibody (NBP2-45743).
 

Pathways for ERK4/MAPK4 Antibody (NBP2-45743)

View related products by pathway.

PTMs for ERK4/MAPK4 Antibody (NBP2-45743)

Learn more about PTMs related to ERK4/MAPK4 Antibody (NBP2-45743).
 

Research Areas for ERK4/MAPK4 Antibody (NBP2-45743)

Find related products by research area.

Blogs on ERK4/MAPK4

There are no specific blogs for ERK4/MAPK4, but you can read our latest blog posts.
mFlour Violet Conjugated Antibodies

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our ERK4/MAPK4 Antibody (OTI2D7) and receive a gift card or discount.

Bioinformatics

Gene Symbol MAPK4