ERK2 Antibody (1D1)

Western Blot: ERK2 Antibody (1D1) [H00005594-M01] - MAPK1 monoclonal antibody (M01), clone 1D1 Analysis of MAPK1 expression in Hela S3 NE.

Immunocytochemistry/Immunofluorescence: ERK2 Antibody (1D1) [H00005594-M01] - Analysis of monoclonal antibody to MAPK1 on HeLa cell. Antibody concentration 10 ug/ml.

ELISA: ERK2 Antibody (1D1) [H00005594-M01] - Detection limit for recombinant GST tagged MAPK1 is approximately 0.1ng/ml as a capture antibody.

Proximity Ligation Assay: ERK2 Antibody (1D1) [H00005594-M01] - Analysis of protein-protein interactions between TP53 and MAPK1. HeLa cells were stained with anti-TP53 rabbit purified polyclonal 1:1200 and anti-MAPK1 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).

Proximity Ligation Assay: ERK2 Antibody (1D1) [H00005594-M01] - Analysis of protein-protein interactions between MAPK3 and MAPK1. Huh7 cells were stained with anti-MAPK3 rabbit purified polyclonal 1:1200 and anti-MAPK1 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).

Sandwich ELISA: ERK2 Antibody (1D1) [H00005594-M01] - Detection limit for recombinant GST tagged MAPK1 is approximately 0.03ng/ml as a capture antibody.

Product Discontinued

ERK2 Antibody (1D1) Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: MAPK1 (AAH17832, 261 a.a. ~ 360 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. RNYLLSLPHKNKVPWNRLFPNADSKALDLLDKMLTFNPHKRIEVEQALAHPYLEQYYDPSDEPIAEAPFKFDMELDDLPKEKLKELIFEETARFQPGYRS
• Specificity: MAPK1 - mitogen-activated protein kinase 1
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: MAPK1
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Proximity Ligation Assay
  • Sandwich ELISA
  • Western Blot 1:500
• Application Notes: Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IF and ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for ERK2 Antibody (1D1)

• EC 2.7.11
• EC 2.7.11.24
• ERK
• ERK2
• ERK-2
• ERK2MAP kinase isoform p42
• ERT1
• Extracellular signal-regulated kinase 2
• MAP kinase 1
• MAP kinase 2
• MAPK 1
• MAPK1
• MAPK2
• mitogen-activated protein kinase 1
• Mitogen-activated protein kinase 2
• p38
• p40
• p41
• p41mapk
• p42mapk
• p42-MAPK
• PRKM1
• PRKM1MAPK 2
• PRKM2
• protein tyrosine kinase ERK2

Background

The protein encoded by this gene is a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. The activation of this kinase requires its phosphorylation by upstream kinases. Upon activation, this kinase translocates to the nucleus of the stimulated cells, where it phosphorylates nuclear targets. Two alternatively spliced transcript variants encoding the same protein, but differing in the UTRs, have been reported for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: MAPK1
• Entrez:
  • 5594(Human)
• OMIM:
  • 176948(Human)
• Uniprot:
  • AAH17832(Human)