ERK1/ERK2 Antibody (216703) [Unconjugated]

Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, PC-12 rat adrenal pheochromocytoma cell line, and TS1 mouse helper T cell line. PVDF membrane was probed with 0.5 µg/mL Mouse Anti-Human/Mouse/Rat ERK1/ERK2 Monoclonal Antibody (Catalog # MAB1576) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). For additional reference, recombinant human ERK1 and ERK2 (2 ng/lane) were included. A specific band for ERK1/ERK2 was detected at approximately 44/42 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 4.

ERK1/ERK2 was detected in immersion fixed paraffin-embedded sections of human brain (cortex) using Mouse Anti-Human/Mouse/Rat ERK1/ERK2 Monoclonal Antibody (Catalog # MAB1576) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal cell bodies. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

• Reactivity: Hu, Mu, Rt
• Applications: WB, IHC
• Clone: 216703
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

ERK1/ERK2 Antibody (216703) [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human ERK1
  Met1-Pro379
  Accession # P27361
• Specificity: Detects human, mouse, and rat ERK1/ERK2 in Western blots.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 8-25 ug/mL
  • Western Blot 0.5 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for ERK1/ERK2 Antibody (216703) [Unconjugated]

• ERK1
• ERK-1
• ERK1/ERK2
• ERT2
• Extracellular signal-regulated kinase 1
• extracellular signal-related kinase 1
• HS44KDAP
• HUMKER1A
• Insulin-stimulated MAP2 kinase
• MAPK 1
• Microtubule-associated protein 2 kinase
• mitogen-activated protein kinase 3
• p44erk1
• p44-ERK1
• p44mapk
• p44-MAPK
• PRKM3

Background

ERK1 and ERK2 (also known as MAPK3 and MAPK1) are 44 and 42 kDa Ser/Thr kinases, respectively. They are part of the Ras-Raf ERK signal transduction cascade often found downstream of growth factor receptor activation. ERK1 and ERK2 were initially isolated and cloned as kinases activated in response to insulin and NGF. They are expressed in most, if not all, mammalian tissues. Dual threonine and tyrosine phosphorylation activates both ERKs, at Thr202/Tyr204 for human ERK1 and Thr185/Tyr187 for human ERK2. The two proteins share 83% amino acid identity, differing mainly at the N and C termini.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ERK1/ERK2 Antibody (MAB1576)(19)

Publications using MAB1576

• Bharadwaj, VN;Klukinov, M;Cowan, RP;Mahinparvar, N;Clark, DJ;Yeomans, DC; Oxytocin Receptors on Calvarial Periosteal Innervation: Therapeutic Target for Post-Traumatic Headache? Pharmaceutics 2024-06-04 [PMID: 38931882] (Immunohistochemistry, Rat)
• Qian Xia, Meng Mao, Gaofeng Zhan, Zhenzhao Luo, Yin Zhao, Xing Li SENP3-mediated deSUMOylation of c-Jun facilitates microglia-induced neuroinflammation after cerebral ischemia and reperfusion injury iScience 2023-06-16 [PMID: 37332598] (Western Blot, Mouse)
• Parkyong Song, Seo Young Choi, Ji Sun Hwang, Hyeon Cheal Park, Keun Ki Kim, Hong-Joo Son et al. Chrysanthemum boreale Makino Inhibits Oxidative Stress-Induced Neuronal Damage in Human Neuroblastoma SH-SY5Y Cells by Suppressing MAPK-Regulated Apoptosis Molecules 2022-08-26 [PMID: 36080264]
• M ?witalska, B Filip-Psur, M Milczarek, M Psurski, A Moszy?ska, AM D?browska, M Gawro?ska, K Krzymi?ski, M Bagi?ski, R Bartoszews, J Wietrzyk Combined anticancer therapy with imidazoacridinone C-1305 and paclitaxel in human lung and colon cancer xenografts-Modulation of tumour angiogenesis Oncogene, 2022-06-14;0(0):. 2022-06-14 [PMID: 35701366] (Simple Western, Xenograft)
• Satoshi Narahara, Takehisa Watanabe, Katsuya Nagaoka, Nahoko Fujimoto, Yoki Furuta, Kentaro Tanaka et al. Clusterin and Related Scoring Index as Potential Early Predictors of Response to Sorafenib in Hepatocellular Carcinoma Hepatology Communications 2022-05-01 [PMID: 34837478]
• E Soleimanpo, JR Bergado Ac, P Landgraf, D Mayer, E Dankert, DC Dieterich, M Fendt Regulation of CREB Phosphorylation in Nucleus Accumbens after Relief Conditioning Cells, 2021-01-26;10(2):. 2021-01-26 [PMID: 33530478] (Western Blot, Rat)
• Ana I. Sánchez, Mary A. García-Acero, Angela Paredes, Rossi Quero, Rita I. Ortega, Jorge A. Rojas et al. Immunodeficiency in a Patient with 22q11.2 Distal Deletion Syndrome and a p.Ala7dup Variant in the <b><i>MAPK1</i></b> Gene Molecular Syndromology 2020-01-01 [PMID: 32256297]
• O Beck, C Paret, A Russo, J Burhenne, M Fresnais, K Steimel, L Seidmann, DC Wagner, N Vewinger, N Lehmann, M Sprang, N Backes, L Roth, MA Neu, A Wingerter, N Henninger, KE Malki, H Otto, F Alt, A Desuki, T Kindler, J Faber Safety and Activity of the Combination of Ceritinib and Dasatinib in Osteosarcoma Cancers (Basel), 2020-03-26;12(4):. 2020-03-26 [PMID: 32224911] (Functional Assay, Human)
• N Vewinger, S Huprich, L Seidmann, A Russo, F Alt, H Bender, C Sommer, D Samuel, N Lehmann, N Backes, L Roth, PN Harter, K Filipski, J Faber, C Paret IGF1R Is a Potential New Therapeutic Target for HGNET-BCOR Brain Tumor Patients Int J Mol Sci, 2019-06-21;20(12):. 6/21/2019 [PMID: 31234291]
• Gennaro Napolitano, Alessandra Esposito, Heejun Choi, Maria Matarese, Valerio Benedetti, Chiara Di Malta et al. mTOR-dependent phosphorylation controls TFEB nuclear export Nature Communications 2018-08-17 [PMID: 30120233]
• Katoh Shinsuke, Kobayashi Junya, Umeda Tomonobu, Kobayashi Yoshiko, Nobuo Izumo, Suzuki Takahiko Chronic irradiation with low-dose-rate 137Cs-gamma rays inhibits NGF-induced neurite extension of PC12 cells via Ca2+/calmodulin-dependent kinase II activation Journal of Radiation Research 2017-11-01 [PMID: 29106600]
• Adriana Carol Eleonora Graziano, Venera Cardile, Rosanna Avola, Nunzio Vicario, Carmela Parenti, Lucia Salvatorelli et al. Wilms' tumor gene 1 silencing inhibits proliferation of human osteosarcoma MG-63 cell line by cell cycle arrest and apoptosis activation Oncotarget 2017-02-21 [PMID: 28107196]
• Non-neuronal cholinergic system contributes to corticosteroid resistance in chronic obstructive pulmonary disease patients Respir. Res., 2016-11-08;17(1):145. 11/8/2016 [PMID: 27825347]
• Sirtuin 3 (SIRT3) Regulates ?-Smooth Muscle Actin (?-SMA) Production through the Succinate Dehydrogenase-G Protein-coupled Receptor 91 (GPR91) Pathway in Hepatic Stellate Cells J Biol Chem, 2016-02-24;291(19):10277-92. 2016-02-24 [PMID: 26912655] (Western Blot, Human)
• Ahmed M. Abu El-Asrar, Mohammad Mairaj Mairaj Siddiquei, Mohd Imtiaz Nawaz, Karel Geboes, Ghulam Mohammad The Proinflammatory Cytokine High-Mobility Group Box-1 Mediates Retinal Neuropathy Induced by Diabetes Mediators of Inflammation 2014-03-10 [PMID: 24733965]
• Lo H, Chen C, Yang C, Wu P, Tsou C, Chiang K, Wu W The carotenoid lutein enhances matrix metalloproteinase-9 production and phagocytosis through intracellular ROS generation and ERK1/2, p38 MAPK, and RARbeta activation in murine macrophages. J Leukoc Biol, 2013-02-19;93(5):723-35. 2013-02-19 [PMID: 23431043] (Western Blot, Mouse)
• Clarke D, Al Ahmad A, Lee B, Parham C, Auckland L, Fertala A, Kahle M, Shaw C, Roberts J, Bix G Perlecan Domain V induces VEGF secretion in brain endothelial cells through integrin alpha5beta1 and ERK-dependent signaling pathways. PLoS ONE, 2012-09-17;7(9):e45257. 2012-09-17 [PMID: 23028886] (Western Blot, Human)
• Lane AE, Tan JT, Hawkins CL The myeloperoxidase-derived oxidant HOSCN inhibits protein tyrosine phosphatases and modulates cell signalling via the mitogen-activated protein kinase (MAPK) pathway in macrophages. Biochem. J., 2010-08-15;430(1):161-9. 2010-08-15 [PMID: 20528774] (Western Blot, Mouse)
• Han Y, Wu G, Deng J, Tao J, Guo L, Tian X, Kang J, Zhang X, Yan C Cellular repressor of E1A-stimulated genes inhibits human vascular smooth muscle cell apoptosis via blocking P38/JNK MAP kinase activation. J. Mol. Cell. Cardiol., 2010-01-06;48(6):1225-35. 2010-01-06 [PMID: 20060003] (Western Blot, Human)

Reviews for ERK1/ERK2 Antibody (MAB1576) (3)

Average Rating: 4

(Based on 3 reviews)

We have 3 reviews tested in 2 species: Human, Rat.

reviewed by: Verified Customer WB Rat 02/10/2015

Summary

• Application: Western Blot
• Sample Tested: See PMID 22818713
• Species: Rat

reviewed by: Verified Customer WB Human 02/10/2015

Summary

• Application: Western Blot
• Sample Tested: See PMID 25255977
• Species: Human

reviewed by: Verified Customer WB Human 02/10/2015

Summary

• Application: Western Blot
• Sample Tested: See PMID 24888374
• Species: Human

Bioinformatics

• Uniprot:
  • P27361()