ERK1/ERK2 Antibody (216703) [Unconjugated]

Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, PC-12 rat adrenal pheochromocytoma cell line, and TS1 mouse helper T cell line. PVDF membrane was probed with 0.5 µg/mL Mouse Anti-Human/Mouse/Rat ERK1/ERK2 Monoclonal Antibody (Catalog # MAB1576) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). For additional reference, recombinant human ERK1 and ERK2 (2 ng/lane) were included. A specific band for ERK1/ERK2 was detected at approximately 44/42 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 4.

ERK1/ERK2 was detected in immersion fixed paraffin-embedded sections of human brain (cortex) using Mouse Anti-Human/Mouse/Rat ERK1/ERK2 Monoclonal Antibody (Catalog # MAB1576) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal cell bodies. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

• Reactivity: Hu, Mu, Rt
• Applications: WB, IHC
• Clone: 216703
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

ERK1/ERK2 Antibody (216703) [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human ERK1
  Met1-Pro379
  Accession # P27361
• Specificity: Detects human, mouse, and rat ERK1/ERK2 in Western blots.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Purity: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot 0.5 ug/mL
  • Immunohistochemistry 8-25 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Protein A or G purified from hybridoma culture supernatant
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for ERK1/ERK2 Antibody (216703) [Unconjugated]

• ERK1/ERK2

Background

ERK1 and ERK2 (also known as MAPK3 and MAPK1) are 44 and 42 kDa Ser/Thr kinases, respectively. They are part of the Ras-Raf ERK signal transduction cascade often found downstream of growth factor receptor activation. ERK1 and ERK2 were initially isolated and cloned as kinases activated in response to insulin and NGF. They are expressed in most, if not all, mammalian tissues. Dual threonine and tyrosine phosphorylation activates both ERKs, at Thr202/Tyr204 for human ERK1 and Thr185/Tyr187 for human ERK2. The two proteins share 83% amino acid identity, differing mainly at the N and C termini.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ERK1/ERK2 Antibody (MAB1576)(5)

Publications using MAB1576

• Sirtuin 3 (SIRT3) Regulates ?-Smooth Muscle Actin (?-SMA) Production through the Succinate Dehydrogenase-G Protein-coupled Receptor 91 (GPR91) Pathway in Hepatic Stellate Cells J Biol Chem, 2016;291(19):10277-92. 2016 [PMID: 26912655] (Western Blot, Human)
• Lo H, Chen C, Yang C, Wu P, Tsou C, Chiang K, Wu W The carotenoid lutein enhances matrix metalloproteinase-9 production and phagocytosis through intracellular ROS generation and ERK1/2, p38 MAPK, and RARbeta activation in murine macrophages. J Leukoc Biol, 2013;93(5):723-35. 2013 [PMID: 23431043] (Western Blot, Mouse)
• Clarke D, Al Ahmad A, Lee B, Parham C, Auckland L, Fertala A, Kahle M, Shaw C, Roberts J, Bix G Perlecan Domain V induces VEGF secretion in brain endothelial cells through integrin alpha5beta1 and ERK-dependent signaling pathways. PLoS ONE, 2012;7(9):e45257. 2012 [PMID: 23028886] (Western Blot, Human)
• Lane AE, Tan JT, Hawkins CL The myeloperoxidase-derived oxidant HOSCN inhibits protein tyrosine phosphatases and modulates cell signalling via the mitogen-activated protein kinase (MAPK) pathway in macrophages. Biochem. J., 2010;430(1):161-9. 2010 [PMID: 20528774] (Western Blot, Mouse)
• Han Y, Wu G, Deng J, Tao J, Guo L, Tian X, Kang J, Zhang X, Yan C Cellular repressor of E1A-stimulated genes inhibits human vascular smooth muscle cell apoptosis via blocking P38/JNK MAP kinase activation. J. Mol. Cell. Cardiol., 2010;48(6):1225-35. 2010 [PMID: 20060003] (Western Blot, Human)

Reviews for ERK1/ERK2 Antibody (MAB1576) (3)

Average Rating: 4

(Based on 3 reviews)

We have 3 reviews tested in 2 species: Human, Rat.

reviewed by: Anonymous WB Rat 02/10/2015

Summary

• Application: Western Blot
• Sample Tested: See PMID 22818713
• Species: Rat

Blocking

• Blocking Details: See PMID 22818713

Details

• Detection Notes: See PMID 22818713

Comments

• Comments: Independent review from antibodyresource.comPublished in PMID 22818713

reviewed by: Anonymous WB Human 02/10/2015

Summary

• Application: Western Blot
• Sample Tested: See PMID 25255977
• Species: Human

Blocking

• Blocking Details: See PMID 25255977

Details

• Detection Notes: See PMID 25255977

Comments

• Comments: Independent review from antibodyresource.comPublished in PMID 25255977

reviewed by: Anonymous WB Human 02/10/2015

Summary

• Application: Western Blot
• Sample Tested: See PMID 24888374
• Species: Human

Blocking

• Blocking Details: See PMID 24888374

Details

• Detection Notes: See PMID 24888374

Comments

• Comments: Independent review from antibodyresource.comPublished in PMID 24888374.

Bioinformatics

• Uniprot:
  • P27361()