Human ERK1/2 [p Tyr187, p Thr202, p Tyr204, p Thr185] ELISA Kit

Product Discontinued

Human ERK1/2 [p Tyr187, p Thr202, p Tyr204, p Thr185] ELISA Kit Summary

• Description: MAPK3 (pT202/Y204)/MAPK1 (pT185/Y187) (Human) ELISA Kit is an enzyme-linked immunosorbent assay for the qualitative determination of human phospho MAPK3 (T202/Y204) and phospho MAPK1 (T185/Y187).
• Modification: p Tyr187, p Thr202, p Tyr204, p Thr185
• Sample Volume: 100 uL
• Kit Type: ELISA Kit
• Gene: MAPK3

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: Useful for qualification.

Packaging, Storage & Formulations

• Storage: Store at -20 °C.

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Human ERK1/2 [p Tyr187, p Thr202, p Tyr204, p Thr185] ELISA Kit

• EC 2.7.11
• ERK-1
• ERK1p44-MAPK
• ERT2
• Extracellular signal-regulated kinase 1
• extracellular signal-related kinase 1
• HS44KDAP
• HUMKER1A
• Insulin-stimulated MAP2 kinase
• MAP kinase 1
• MAP kinase 3
• MAP kinase isoform p44
• MAPK 1
• MAPK 3
• MGC20180
• Microtubule-associated protein 2 kinase
• Mitogen-activated protein kinase 1
• mitogen-activated protein kinase 3
• p44erk1
• p44-ERK1
• p44mapk
• PRKM3EC 2.7.11.24

Background

The extracellular signal-regulated kinases 1 and 2 (ERK1 and ERK2), also called p44 and p42 MAP kinases, are members of the Mitogen Activated Protein Kinase (MAPK) family of proteins found in all eukaryotes. Because the 44 kDa ERK1 and the 42 kDa ERK2 are highly homologous and both function in the same protein kinase cascade, the two proteins are often referred to collectively as ERK1/2 or p44/p42 MAP kinase (1). They are both located in the cytosol and mitochondria (2). While the role of cytosol ERK1/2 is well studied and involved in multiple cellular functions (2), the role of mitochondrial ERK1/2 remains poorly understood. Both ERK 1 and 2 are activated by MEK1 or MEK2, by dual phosphorylation of a threonine and tyrosine residue in the activation loop (TEY motif) (1, 3). Either phosphorylation alone can induce an electrophoretic mobility shift, but both are required for activation of the kinase. This dual phosphorylation is efficiently detected by phosphorylation state-specific antibody directed to the pTEpY motif. Once activated, MAP kinases phosphorylate a broad spectrum of substrates, including cytoskeletal proteins, translation regulators, transcription factors, and the Rsk family of protein kinases (4). ERK1/2 activation is generally thought to confer a survival advantage to cells (5); however there is increasing evidence that suggests that the activation of ERK1/2 also contributes to cell death under certain conditions (5). ERK1/2 also is activated in neuronal and renal epithelial cells upon exposure to oxidative stress and toxicants or deprivation of growth factors, and inhibition of the ERK pathway blocks apoptosis (5).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

FAQs for ERK1/2 ELISA Kit (KA2163). (Showing 1 - 1 of 1 FAQ).

1. wondering what the difference is between your quantikine and duo set elisas?
   • Usually the duosets do not have the entire kit such as plates and buffers, whereas the other kits are complete.

Bioinformatics

• Gene Symbol: MAPK3