ERK1/2 [p Thr202, p Tyr204] Antibody

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ERK1/2 [p Thr202, p Tyr204] Antibody Summary

• Immunogen: Synthetic phosphopeptide derived from human ERK1/2 around the phosphorylation site of Threonine 202/Tyrosine 204.
• Modification: p Thr202, p Tyr204
• Clonality: Polyclonal
• Host: Rabbit
• Gene: MAPK3
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:500-1:1000
• Application Notes: Western blot (WB) analysis of p-ERK1/2 (T202/Y204) pAb in extracts from Jurkat PMA cells .
  Immunohistochemistry (IHC) analyzes of p-ERK1/2 (T202/Y204) pAb in paraffin-embedded human breast carcinoma tissue.
• Theoretical MW: 42 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2)
• Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for ERK1/2 [p Thr202, p Tyr204] Antibody

• EC 2.7.11
• ERK-1
• ERK1p44-MAPK
• ERT2
• Extracellular signal-regulated kinase 1
• extracellular signal-related kinase 1
• HS44KDAP
• HUMKER1A
• Insulin-stimulated MAP2 kinase
• MAP kinase 1
• MAP kinase 3
• MAP kinase isoform p44
• MAPK 1
• MAPK 3
• MGC20180
• Microtubule-associated protein 2 kinase
• Mitogen-activated protein kinase 1
• mitogen-activated protein kinase 3
• p44erk1
• p44-ERK1
• p44mapk
• PRKM3EC 2.7.11.24

Background

The activation of signal transduction pathways by growth factors, hormones and neurotransmitters is mediated through two closely related MAP kinases, p44 and p42, designated extracellular-signal related kinase 1 (ERK 1) and ERK 2, respectively. ERK proteins are regulated by dual phosphorylation at Tyrosine 204 and 187 and Threonine 177 and 160 residues mapping within a characteristic Thr-Glu-Tyr motif. Phosphorylation at both the Threonine 202 and Tyrosine 204 residues of ERK 1 and Threonine 185 and Tyrosine 187 residues of ERK 2 is required for full enzymatic activation. The structural consequences of dual phosphorylation in ERK 2 include active site closure, alignment of key catalytic residues that interact with ATP, and remodeling of the activation loop. In response to activation, MAP kinases phosphorylate downstream components on serine and threonine. Upstream MAP kinase regulators include MAP kinase kinase (MEK), MEK kinase and Raf-1. The ERK family has three additional members: ERK 3, ERK 5 and ERK 6.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ERK1/2 Antibody (NBP1-19924)(4)

Publications using NBP1-19924

• Lu H Samanta D Xiang L et al. Chemotherapy triggers HIF-1-dependent glutathione synthesis and copper chelation that induces the breast cancer stem cell phenotype. Proc Natl Acad Sci U S A. 2015-07-30 [PMID: 26229077] (WB, Human)
• Huang CP, Cheng CM, Su HL, Lin YW. Syndecan-4 Promotes Epithelial Tumor Cells Spreading and Regulates the Turnover of PKCa Activity under Mechanical Stimulation on the Elastomeric Substrates. Cell. Physiol. Biochem. 2015-07-01 [PMID: 26160374]
• Jhang JJ, Cheng YT, Ho CY, Yen, GC. Monosodium urate crystals trigger Nrf2- and heme oxygenase-1-dependent inflammation in THP-1 cells. Cell. Mol. Immunol. 2014-08-11 [PMID: 25109682]
• Chen WH, Cheng SJ, Tzen JT et al. Probing Relevant Molecules in Modulating the Neurite Outgrowth of Hippocampal Neurons on Substrates of Different Stiffness. PLoS One 2013-12-30 [PMID: 24386192] (WB, Mouse)

Reviews for ERK1/2 Antibody (NBP1-19924) (3)

Average Rating: 4.7

(Based on 3 reviews)

We have 3 reviews tested in 1 species: Human.

reviewed by: Verified Customer WB Human 10/22/2018

Summary

• Application: Western Blot
• Sample Tested: Human breast cancer cell lines
• Species: Human
• Lot: CH34141

reviewed by: Verified Customer WB Human 09/07/2015

Summary

• Application: Western Blot
• Sample Tested: Human cancer cell whole cell lysate
• Species: Human

reviewed by: Verified Customer WB Human 01/05/2012

Summary

• Application: Western Blot
• Sample Tested: Hela cell line
• Species: Human

FAQs for ERK1/2 Antibody (NBP1-19924). (Showing 1 - 1 of 1 FAQs).

1. I would like to ensure that the NBP1-19924 recognizes only ERK1, or ERK1/2 [p Thr202, p Tyr204].
   • I can confirm you that this antibody will detect both ERK1 (phosphorylated at Thr202 and Tyr204) and ERK2 (phosphorylated at Thr185 and Tyr187). ERK1 and ERK2 are very similar in sequence around ERK1's phospho site Thr202/Tyr204 and the latter is identical with phospho sites Thr185 /Tyr187 in ERK2.

Bioinformatics

• Gene Symbol: MAPK3
• Entrez:
  • 5595(Human)
  • 26417(Mouse)
  • 50689(Rat)
• Uniprot:
  • P27361()