ERK1/2 Antibody

Product Discontinued

ERK1/2 Antibody Summary

• Immunogen: Synthetic peptide, corresponding to amino acids 320-370 of Human ERK1.
• Clonality: Polyclonal
• Host: Rabbit
• Gene: MAPK3
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:500-1:1000
• Application Notes: Western blot (WB) analysis of ERK1/2 (L352) pAb in extracts from COLO205 cells.
  Immunohistochemistry (IHC) analyzes of ERK1/2 (L352) pAb in paraffin-embedded human breast carcinoma tissue.
• Theoretical MW: 42 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Human, Mouse and Rat.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2)
• Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for ERK1/2 Antibody

• EC 2.7.11
• ERK-1
• ERK1p44-MAPK
• ERT2
• Extracellular signal-regulated kinase 1
• extracellular signal-related kinase 1
• HS44KDAP
• HUMKER1A
• Insulin-stimulated MAP2 kinase
• MAP kinase 1
• MAP kinase 3
• MAP kinase isoform p44
• MAPK 1
• MAPK 3
• MGC20180
• Microtubule-associated protein 2 kinase
• Mitogen-activated protein kinase 1
• mitogen-activated protein kinase 3
• p44erk1
• p44-ERK1
• p44mapk
• PRKM3EC 2.7.11.24

Background

The activation of signal transduction pathways by growth factors, hormones and neurotransmitters is mediated through two closely related MAP kinases, p44 and p42, designated extracellular-signal related kinase 1 (ERK 1) and ERK 2, respectively. ERK proteins are regulated by dual phosphorylation at specific tyrosine and Threonine sites mapping within a characteristic Thr- Glu-Tyr motif. Phosphorylation at both the Thr and Tyr residues is required for full enzymatic activation. In response to activation, MAP kinases phos- phorylate downstream components on Serine and Threonine. Upstream MAP kinase regulators include MAP kinase kinase (MEK), MEK kinase and Raf-1. The ERK family has three additional members: ERK 3, ERK 5 and ERK 6.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: MAPK3
• Entrez:
  • 5595(Human)
  • 50689(Rat)