ERK1/2 [p Thr202, p Thr185] Antibody (SZ2-4)

Immunocytochemistry/Immunofluorescence: ERK1/2 [p Thr202, p Thr185] Antibody (SZ2-4) [NBP2-67359] - Staining Phospho-Erk1(T202)+Erk2(T185) in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X-100/PBS.

Immunohistochemistry-Paraffin: ERK1/2 [p Thr202, p Thr185] Antibody (SZ2-4) [NBP2-67359] - Analysis of paraffin-embedded human gallbladder tissue with Rabbit anti-ERK1/2[p Thr202, pThr185] antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Flow Cytometry: ERK1/2 [p Thr202, p Thr185] Antibody (SZ2-4) [NBP2-67359] - Analysis of MCF-7 cells with Phospho-Erk1(T202)+Erk2(T185) antibody at 1:50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as secondary antibody.

Immunocytochemistry/Immunofluorescence: ERK1/2 [p Thr202, p Thr185] Antibody (SZ2-4) [NBP2-67359] - Staining Phospho-Erk1(T202)+Erk2(T185) in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunocytochemistry/Immunofluorescence: ERK1/2 [p Thr202, p Thr185] Antibody (SZ2-4) [NBP2-67359] - Staining Phospho-Erk1(T202)+Erk2(T185) in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X-100/PBS.

Immunohistochemistry-Paraffin: ERK1/2 [p Thr202, p Thr185] Antibody (SZ2-4) [NBP2-67359] - Analysis of paraffin-embedded human kidney tissue using anti-Phospho-Erk1(T202)+Erk2(T185) antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: ERK1/2 [p Thr202, p Thr185] Antibody (SZ2-4) [NBP2-67359] - Staining in canine epidermis. Antigen retrieval: citrate buffer (pH 6.0) for 10 minutes at 121C. Blocking of nonspecific reactions in 3% hydrogen peroxide in methanol at room temperature for 5min, followed by 8% skimmed milk at 37C for 40min. Primary antibody dilution: 1:100 and Secondary antibody: Dako EnVision + System. Reaction products were visualized with 0.05% 3.3'-diaminobenzidine (DAB) and 0.03% hydrogen peroxide in Tris-hydrochloric buffer and then counterstained with Mayer's hematoxylin. IHC-P image submitted by verified customer review.

Immunohistochemistry-Paraffin: ERK1/2 [p Thr202, p Thr185] Antibody (SZ2-4) [NBP2-67359] - Analysis of paraffin-embedded human lung cancer tissue using anti-Phospho-Erk1(T202)+Erk2(T185) antibody. Counter stained with hematoxylin.

Product Discontinued

ERK1/2 [p Thr202, p Thr185] Antibody (SZ2-4) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Synthetic phospho-peptide corresponding to residues surrounding Thr185 of Human ERK1/2. (SwissProt: P27361 Human; SwissProt: P28482 Human; SwissProt: P63085 Mouse; SwissProt: Q63844 Mouse)
• Modification: p Thr202, p Thr185
• Localization: Cytoplasm, Nucleus, Membrane, Cell junction.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: MAPK3
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Immunocytochemistry/ Immunofluorescence 1:100-1:500
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:1000
• Application Notes: ERK1/2 [p Thr202, p Thr185] antibody validated for IHC-P from a verified customer review.

Reactivity Notes

Canine reactivity reported from a verified customer review.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for ERK1/2 [p Thr202, p Thr185] Antibody (SZ2-4)

• EC 2.7.11
• ERK-1
• ERK1p44-MAPK
• ERT2
• Extracellular signal-regulated kinase 1
• extracellular signal-related kinase 1
• HS44KDAP
• HUMKER1A
• Insulin-stimulated MAP2 kinase
• MAP kinase 1
• MAP kinase 3
• MAP kinase isoform p44
• MAPK 1
• MAPK 3
• MGC20180
• Microtubule-associated protein 2 kinase
• Mitogen-activated protein kinase 1
• mitogen-activated protein kinase 3
• p44erk1
• p44-ERK1
• p44mapk
• PRKM3EC 2.7.11.24

Background

The extracellular signal-regulated kinases 1 and 2 (ERK1 and ERK2), also called p44 and p42 MAP kinases, are members of the Mitogen Activated Protein Kinase (MAPK) family of proteins found in all eukaryotes. Because the 44 kDa ERK1 and the 42 kDa ERK2 are highly homologous and both function in the same protein kinase cascade, the two proteins are often referred to collectively as ERK1/2 or p44/p42 MAP kinase (1). They are both located in the cytosol and mitochondria (2). While the role of cytosol ERK1/2 is well studied and involved in multiple cellular functions (2), the role of mitochondrial ERK1/2 remains poorly understood. Both ERK 1 and 2 are activated by MEK1 or MEK2, by dual phosphorylation of a threonine and tyrosine residue in the activation loop (TEY motif) (1, 3). Either phosphorylation alone can induce an electrophoretic mobility shift, but both are required for activation of the kinase. This dual phosphorylation is efficiently detected by phosphorylation state-specific antibody directed to the pTEpY motif. Once activated, MAP kinases phosphorylate a broad spectrum of substrates, including cytoskeletal proteins, translation regulators, transcription factors, and the Rsk family of protein kinases (4). ERK1/2 activation is generally thought to confer a survival advantage to cells (5); however there is increasing evidence that suggests that the activation of ERK1/2 also contributes to cell death under certain conditions (5). ERK1/2 also is activated in neuronal and renal epithelial cells upon exposure to oxidative stress and toxicants or deprivation of growth factors, and inhibition of the ERK pathway blocks apoptosis (5).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Review for ERK1/2 Antibody (NBP2-67359) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Canine.

reviewed by: Verified Customer IHC-P Canine 01/10/2020

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: Skin tissue
• Species: Canine
• Lot: HJ1017

Comments

• Comments: ***Bio-Techne Response: This review was submitted through the legacy Novus Innovators Program, reflecting a new species or application tested on a primary antibody.*** Antigen retrieval: citrate buffer (pH 6.0) for 10 minutes at 121 Celcius. Blocking of nonspecific reactions in 3% hydrogen peroxide in methanol at room temperature for 5 minutes, followed by 8% skimmed milk at 37 Celcius for 40 minutes. Primary antibody dilution: 1:100 Secondary antibody: Reaction products were visualized with 0.05% 3.3'-diaminobenzidine (DAB) and 0.03% hydrogen peroxide in Tris-hydrochloric buffer and then counterstained with Mayer's hematoxylin.

Bioinformatics

• Gene Symbol: MAPK3