ERG28 Overexpression Lysate

Images

 
Western Blot: ERG28 Overexpression Lysate (Adult Normal) [NBL1-08166] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for ERG28.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

ERG28 Overexpression Lysate Summary

Description
Transient overexpression lysate of chromosome 14 open reading frame 1 (C14orf1) The lysate was created in HEK293T cells, using Plasmid ID RC200238 and based on accession number NM_007176. The protein contains a C-MYC/DDK Tag.
Gene
C14ORF1

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot.

You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul).
Theoretical MW
15.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Array

Type
Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for ERG28 Overexpression Lysate

  • chromosome 14 open reading frame 1
  • ERG28
  • NET51
  • probable ergosterol biosynthetic protein 28

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP2-17029
Species: Hu, Mu, Rt
Applications: WB
NBP2-55973
Species: Hu
Applications: WB, ICC/IF
NBP2-84125
Species: Hu
Applications: WB, IHC, IHC-P
NB100-2322
Species: Hu, Mu, Rt, Bv, Ca, Rb, Sh
Applications: WB, Simple Western, ELISA, Flow, ICC/IF, IHC, IHC-P, Flow-IC, KD
NB600-302
Species: Hu, Mu, Bv, Dr
Applications: WB, Simple Western, ChIP, ELISA, Flow, IB, ICC/IF, IHC, IHC-Fr, IHC-P, IP, PLA, S-ELISA, Flow-IC
NBP2-60655
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-59450
Species: Hu
Applications: WB
NBP3-03109
Species: Hu, Mu, Rt
Applications: WB, IHC, IHC-P
NB100-116
Species: Hu, Mu, Rt, Pm
Applications: WB, Simple Western, ChIP, ELISA, GS, IB, ICC/IF, IHC, IHC-Fr, IHC-P, IP, PLA, ChIP, KD
NBP2-89114
Species: Hu, Mu, Rt
Applications: WB, Flow, ICC/IF, IHC, IHC-P
NB110-74569
Species: Hu, Mu, Rt
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP
NBP2-45731
Species: Hu, Ca, Pm
Applications: WB, IHC, IHC-P
NBP1-86265
Species: Hu
Applications: WB, IHC, IHC-P
NBP2-34032
Species: Hu
Applications: IHC, IHC-P
NBL1-08166
Species: Hu
Applications: WB

Publications for ERG28 Lysate (NBL1-08166) (0)

There are no publications for ERG28 Lysate (NBL1-08166).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for ERG28 Lysate (NBL1-08166) (0)

There are no reviews for ERG28 Lysate (NBL1-08166). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for ERG28 Lysate (NBL1-08166) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Other Available Formats

NBL1-08166

Additional ERG28 Products

Bioinformatics Tool for ERG28 Lysate (NBL1-08166)

Discover related pathways, diseases and genes to ERG28 Lysate (NBL1-08166). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for ERG28 Lysate (NBL1-08166)

Discover more about diseases related to ERG28 Lysate (NBL1-08166).
 

Pathways for ERG28 Lysate (NBL1-08166)

View related products by pathway.

Blogs on ERG28

There are no specific blogs for ERG28, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our ERG28 Overexpression Lysate and receive a gift card or discount.

Bioinformatics

Gene Symbol C14ORF1