ERCC6L Antibody (3F12-2B10) - Azide and BSA Free

Western Blot: ERCC6L Antibody (3F12-2B10) [H00054821-M01] - Analysis of ERCC6L expression in transfected 293T cell line by ERCC6L monoclonal antibody (M01), clone 3F12-2B10.Lane 1: ERCC6L transfected lysate(46.2 KDa).Lane 2: Non-transfected lysate.

ELISA: ERCC6L Antibody (3F12-2B10) [H00054821-M01] - Detection limit for recombinant GST tagged ERCC6L is approximately 0.03ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF
• Clone: 3F12-2B10
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

ERCC6L Antibody (3F12-2B10) - Azide and BSA Free Summary

• Immunogen: ERCC6L (AAH08808, 1 a.a. ~ 419 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MEKSFATKNEAVQKETLQEGPKQEALQEDPLESFNYVLSKSTKADIGPNLDQLKDDEILRHCNPWPIISITNESQNAESNVSIIEIADDLSASHSALQDAQASEAKLEEEPSASSPQYACDFNLFLEDSADNRQNFSSQSLEHVEKENSLCGSAPNSRAGFVHSKTCLSWEFSEKDDEPEEVVVKAKIRSKARRIVSDGEDEDDSFKDTSSINPFNTSLFQFSSVKQFDASTPKNDISPPGRFFSSQIPSSVNKSMNSRRSLASRRSLINMVLDHVEDMEERLDDSSEAKGPEDYPEEGVEESSGEASKYTEEDPSGETLSSENKSSWLMTSKPSALAQETSLGAPEPLSGEQLVGSPQDKAAEATNDYETLVKRGKELKECGKIQEALNCLVKALDIKSADPEVMLLTLSLYKQLNNN
• Specificity: FLJ20105 - FLJ20105 protein
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: ERCC6L
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence
  • Western Blot 1:500
• Application Notes: Antibody reactive against recombinant protein with GST tag on ELISA and Western Blot. GST tag alone is used as a negative control. Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID : 28904335).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for ERCC6L Antibody (3F12-2B10) - Azide and BSA Free

• ATP-dependent helicase ERCC6-like
• DNA excision repair protein ERCC-6-like
• EC 3.6.1
• EC 3.6.4.12
• excision repair cross-complementing rodent repair deficiency, complementationgroup 6-like
• excision repair protein ERCC6-like
• FLJ20105
• MGC131695
• PICHexcision repair cross-complementing rodent repair deficiency complementationgroup 6 - like
• PLK1-interacting checkpoint helicase
• SNF2/RAD54 family protein
• Tumor antigen BJ-HCC-15

Background

DNA helicase that acts as an essential component of the spindle assembly checkpoint. Contributes to the mitotic checkpoint by recruiting MAD2 to kinetochores and monitoring tension on centromeric chromatin. Acts as a tension sensor that associates with catenated DNA which is stretched under tension until it is resolved during anaphase

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ERCC6L Antibody (H00054821-M01)(7)

Publications using H00054821-M01

• Yan G, Shitian Z, Daizhao D et al. Loss of RanGAP1 drives chromosome instability and rapid tumorigenesis of osteosarcoma. Dev Cell. 2023-01-24 [PMID: 36696903]
• Gemble S, Buhagiar-LabarchEde G, Onclercq-Delic R et al. Topoisomerase II alpha prevents ultrafine anaphase bridges by two mechanisms Open Biol 2020-05-01 [PMID: 32400307]
• Bou Samra E, Buhagiar-Labarchede G, Machon C et al. A role for Tau protein in maintaining ribosomal DNA stability and cytidine deaminase-deficient cell survival. Nat Commun 2017-09-25 [PMID: 28947735]
• Feng W, Jasin M. BRCA2 suppresses replication stress-induced mitotic and G1 abnormalities through homologous recombination Nat Commun 2017-09-13 [PMID: 28904335] (ICC/IF, Human)
• Gemble S, Buhagiar-Labarchede G, Onclercq-Delic R et al. A balanced pyrimidine pool is required for optimal Chk1 activation to prevent ultrafine anaphase bridge formation. J Cell Sci 2016-07-06 [PMID: 27383768]
• Kurasawa Y, Yu-Lee LY. PICH and cotargeted Plk1 coordinately maintain prometaphase chromosome arm architecture. Mol Biol Cell 2010-04-01 [PMID: 20130082]
• Leng M, Bessuso D, Jung SY et al. Targeting Plk1 to chromosome arms and regulating chromosome compaction by the PICH ATPase. Cell Cycle. 2009-05-15 [PMID: 18418076]

Bioinformatics

• Gene Symbol: ERCC6L
• Entrez:
  • 54821(Human)
• Uniprot:
  • AAH08808(Human)