ErbB4/Her4 Antibody (H4.77.16 (Ab77))

Western Blot: ErbB4/Her4 Antibody (H4.77.16) [NB120-3104] - Analysis of 25 ug of mouse brain cell lysates.

Flow Cytometry: ErbB4/Her4 Antibody (H4.77.16) [NB120-3104] - Analysis of HER-4 in T47D cells (green) compared to an isotype control (blue).

Flow Cytometry: ErbB4/Her4 Antibody (H4.77.16) [NB120-3104] - Analysis of HER-4 in MCF-7 cells (green) compared to an isotype control (blue).

Flow Cytometry: ErbB4/Her4 Antibody (H4.77.16) [NB120-3104] - Analysis of HER-4 in Neuro-2a cells (green) compared to an isotype control (blue).

Product Discontinued

ErbB4/Her4 Antibody (H4.77.16 (Ab77)) Summary

• Immunogen: Extracellular fragment of recombinant human c-erbB-4/HER-4 oncoprotein
• Localization: Type I membrane protein
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: ERBB4
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 0.5 ug
  • Immunocytochemistry/ Immunofluorescence 2 ug/ml
  • Immunohistochemistry
  • Immunoprecipitation 2 ug/mg
  • Western Blot 1:50 - 1:500
• Application Notes: Use in Immunohistochemistry reported in scientific literature (PMID 28267150).

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: PBS (pH 7.4) and 0.2% BSA
• Preservative: 0.09% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Protein G purified

Alternate Names for ErbB4/Her4 Antibody (H4.77.16 (Ab77))

• avian erythroblastic leukemia viral (v-erb-b2) oncogene homolog 4
• EC 2.7.10
• EC 2.7.10.1
• ErbB4
• HER4
• HER4MGC138404
• p180erbB4
• Proto-oncogene-like protein c-ErbB-4
• receptor tyrosine-protein kinase erbB-4
• Tyrosine kinase-type cell surface receptor HER4
• v-erb-a avian erythroblastic leukemia viral oncogene homolog-like 4
• v-erb-a erythroblastic leukemia viral oncogene homolog 4 (avian)

Background

C-erbB-4 is the fourth member of class I receptor kinase family (ErbB). Down regulation of ErbB-4 in estrogen receptor-positive cancer cells inhibits proliferation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ErbB4/Her4 Antibody (NB120-3104)(2)

Publications using NB120-3104

• Kosti P, Opzoomer J, Larios-Martinez K et al. Hypoxia-sensing CAR T cells provide safety and efficacy in treating solid tumors Cell Reports Medicine 2021-04-01 [PMID: 33948568] (FLOW)
• Kawata M, Morikawa S, Shiosaka S, Tamura H. Ablation of neuropsin-neuregulin 1 signaling imbalances ErbB4 inhibitory networks and disrupts hippocampal gamma oscillation. Transl Psychiatry. 2017-03-07 [PMID: 28267150] (IF/IHC, Mouse)

Bioinformatics

• Gene Symbol: ERBB4
• Entrez:
  • 2066(Human)
  • 13867(Mouse)
  • 29496(Rat)
• Uniprot:
  • Q15303()