ER beta/NR3A2 Antibody (6A12) - Azide and BSA Free

Western Blot: ER beta/NR3A2 Antibody (6A12) [NB200-303] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Estrogen Receptor beta antibody [6A12]. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.

• Reactivity: Hu
• Applications: WB, ICC/IF, IHC, IP, ChIP
• Clone: 6A12
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

ER beta/NR3A2 Antibody (6A12) - Azide and BSA Free Summary

• Immunogen: Amino acids 1-153 of human ER beta/NR3A2 expressed in E. coli.
• Specificity: This is specific for Estrogen receptor-Beta.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: ESR2
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Chromatin Immunoprecipitation (ChIP)
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry 1:10 - 1:500
  • Immunoprecipitation
  • Western Blot 1-5 ug/ml
• Application Notes: ChIP usage reported in scientific literature.
• Theoretical MW: 55 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: No Preservative
• Purity: Protein G purified

Alternate Names for ER beta/NR3A2 Antibody (6A12) - Azide and BSA Free

• ER beta
• Erb
• ER-beta
• ESR2
• ESR-BETA
• ESTRB
• estrogen receptor 2 (ER beta)
• estrogen receptor beta 4
• estrogen receptor beta
• NR3A2
• NR3A2ESRB
• Nuclear receptor subfamily 3 group A member 2

Background

The human ER-

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ER beta/NR3A2 Antibody (NB200-303)(16)

Publications using NB200-303

• Asavasupreechar T, Chan MSM, Saito R et al. Sex steroid metabolism and actions in non-small cell lung carcinoma J. Steroid Biochem. Mol. Biol. 2019-08-03 [PMID: 31386890] (IF/IHC, Human)
• Andersson S, Sundberg M et al. Insufficient antibody validation challenges oestrogen receptor beta research. Nat Commun 2017-06-15 [PMID: 28643774] (IF/IHC, Human)
• Vivar OI, Zhao X, Saunier EF et al. Estrogen receptor beta binds to and regulates three distinct classes of target genes. J Biol Chem 2010-07-01 [PMID: 20404318] (Chemotaxis, Human)
• Vivar OI, Saunier EF, Leitman DC et al. Selective activation of estrogen receptor-beta target genes by 3,3'-diindolylmethane. Endocrinology 2010-04-01 [PMID: 20160136] (Chemotaxis, Human)
• Cooke, FJ et al. Epifenetic control of the oestrogen receptor genes in breast cancer. Brit J.
• Ivanova MM, Mazhawidza W, Dougherty SM et al. Activity and intracellular location of estrogen receptors alpha and beta in human bronchial epithelial cells. Mol Cell Endocrinol 2009-06-01 [PMID: 19433257] (Chemotaxis, Human)
• Klinge CM, Wickramasinghe NS, Ivanova MM et al. Resveratrol stimulates nitric oxide production by increasing estrogen receptor alpha-Src-caveolin-1 interaction and phosphorylation in human umbilical vein endothelial cells. FASEB J 2008-07-01 [PMID: 18296501] (Chemotaxis, Human)
• Cvoro A, Paruthiyil S, Jones JO et al. Selective activation of estrogen receptor-beta transcriptional pathways by an herbal extract. Endocrinology 2007-02-01 [PMID: 17095596] (Chemotaxis, Human)
• Cvoro A, Tzagarakis-Foster C, Tatomer D et al. Distinct roles of unliganded and liganded estrogen receptors in transcriptional repression. Mol Cell 2006-02-01 [PMID: 16483936] (Chemotaxis, Human)
• Hopp TA, Weiss HL, Parra IS et al. Low levels of estrogen receptor beta protein predict resistance to tamoxifen therapy in breast cancer. Clin Cancer Res 2004-11-01 [PMID: 15569979]
• Tee Primate, Rogatsky I, Tzagarakis-Foster C et al. Estradiol and selective estrogen receptor modulators differentially regulate target genes with estrogen receptors alpha and beta. Mol Biol Cell 2004-03-01 [PMID: 14699072] (Chemotaxis, Human)
• Fuqua, SAW et al. Estrogen receptor beta prtoein in human breast cancer: correlation with clinical tumor parameters. Cancer Res 63:2434-2439. 2003-01-01 [PMID: 14695225]
• Konstatinopoulosa, PA et al. Oestrogen receptor beta (ERb) is abundantly expressed in normal colonic mucosa, but declines in colon adenocarcinoma paralleling the tumor's dedifferentiation. Euro J of Cancer 39:1251-1258. 2003-01-01 [PMID: 12763213]
• Bianco NR, Perry G, Smith MA et al. Functional implications of antiestrogen induction of quinone reductase: inhibition of estrogen-induced deoxyribonucleic acid damage. Mol Endocrinol 2003-07-01 [PMID: 12714703]
• Saji S, Omoto Y, Shimizu C et al. Expression of estrogen receptor (ER) (beta)cx protein in ER(alpha)-positive breast cancer: specific correlation with progesterone receptor. Cancer Res 2002-09-01 [PMID: 12208729]
• Speirs V, Skliris GP, Burdall SE & Carder PJ. Distinct expression patterns of ERa and ERb in normal human mammary gland. J Clin Path, in press. 2002-01-01 [PMID: 11986344]

Bioinformatics

• Gene Symbol: ESR2
• Entrez:
  • 2100(Human)