ER beta/NR3A2 Antibody (1013506) [Unconjugated]

ER beta /NR3A2 was detected in immersion fixed MCF-7 human breast cancer cell line (left panel; positive staining) and THP-1 human acute monocytic leukemia cell line (right panel; negative staining) using Mouse Anti-Human ER beta /NR3A2 Monoclonal Antibody (Catalog # MAB71061) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

• Reactivity: Hu
• Applications: ICC/IF
• Clone: 1013506
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

ER beta/NR3A2 Antibody (1013506) [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human ER beta /NR3A2
  Met1-Gly318
  Accession # Q92731
• Specificity: Detects human ER beta /NR3A2 in direct ELISAs.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 8-25 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for ER beta/NR3A2 Antibody (1013506) [Unconjugated]

• ER beta
• Erb
• ER-beta
• ESR2
• ESR-BETA
• ESTRB
• estrogen receptor 2 (ER beta)
• estrogen receptor beta 4
• estrogen receptor beta
• NR3A2
• NR3A2ESRB
• Nuclear receptor subfamily 3 group A member 2

Background

Estrogen Receptor beta (ER beta ; NR3A2) is a member of the steroid receptor family. The natural ligand for ER is the classical estrogenic compound 17 beta -estradiol. ER beta is expressed in the granulosa cell layer of primary, secondary and mature follicles in the ovary, in bone, bladder, uterus, testis, epididymis, gastrointestinal tract, kidney, breast, heart, vessel wall, immune system, lung, pituitary, hippocampus and hypothalamus. Roles for ER beta in the reproductive and cardiovascular systems have been reported, although these are the subject of conflicting reports. ER beta has been postulated to act primarily as a modulator of ER alpha function. ER beta has been shown to form homodimers as well as heterodimers with ER alpha . Both ER alpha and ER beta can give rise to numerous isoforms.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ER beta/NR3A2 Antibody (MAB71061)(1)

Publication using MAB71061

• Kate A. Wickham, Line B. Nørregaard, Mikkel Oxfeldt, Stephen S. Cheung, Lasse Gliemann, Mette Hansen et al. Short-Term Supplementation With Fermented Red Clover Extract Reduces Vascular Inflammation in Early Post-menopausal Women Frontiers in Cardiovascular Medicine 2022-02-10 [PMID: 35224058] (Western Blot, Human)

Bioinformatics

• Uniprot:
  • Q92731()