Equine IL-1ra/IL-1F3 DuoSet ELISA, 15 Plate

• Reactivity: Eq
• Applications: ELISA
• Conjugate: Biotin

Equine IL-1ra/IL-1F3 DuoSet ELISA, 15 Plate Summary

• Source: N/A
• Assay Type: Solid Phase Sandwich ELISA
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: No significant interference observed with available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Equine IL-1ra/IL-1F3 DuoSet ELISA, 15 Plate

• DIRA
• ICIL-1ra
• IL1F3
• IL-1F3
• IL1ra
• IL-1ra
• IL-1ra3
• IL1RN
• IL-1RN
• interleukin 1 receptor antagonist
• IRAP
• MVCD4

Background

IL-1ra (IL-1 receptor antagonist) is a widely expressed acute phase protein that serves to dampen inflammation. IL-1ra binds to both IL-1 RI and IL-1 RII. It competitively inhibits IL-1 (alpha or beta) binding to IL-1 RI and does not trigger recruitment of the accessory molecule IL-1 R AcP or activation of IL-1 RI signaling. IL-1ra blocks the proinflammatory actions of IL-1 including Prostaglandin E2 and IL-6 release from endothelial cells, fever generation, thrombocytosis, the production of hepatic acute phase proteins, and neutrophil expansion and infiltration.

Publications for IL-1ra/IL-1F3/IL1RN (DY2466)(5)

Publications using DY2466

• Fukuda, K;Mita, H;Tamura, N;Kuroda, T;Kuwano, A;Takahashi, T;Sato, F; Characteristic inflammatory biomarkers in an equine model of persistent synovitis induced by the intra-articular administration of monoiodoacetic acid Journal of equine veterinary science 2023-05-18 [PMID: 37209789] (Equine)
• RF Jiménez-Ar, C López, ME Álvarez, C Giraldo, M Prades, JU Carmona Long-term cytokine and growth factor release from equine platelet-rich fibrin clots obtained with two different centrifugation protocols Cytokine, 2017-06-23;97(0):149-155. 2017-06-23 [PMID: 28648869] (Equine)
• Simon Rütten Release kinetics of tumor necrosis factor-? and interleukin-1 receptor antagonist in the equine whole blood BMC Vet Res, 2016-06-17;12(1):117. 2016-06-17 [PMID: 27316332] (Equine)
• Goodrich L, Grieger J, Phillips J, Khan N, Gray S, McIlwraith C, Samulski R scAAVIL-1ra dosing trial in a large animal model and validation of long-term expression with repeat administration for osteoarthritis therapy. Gene Ther, 2015-04-23;22(7):536-45. 2015-04-23 [PMID: 25902762] (Equine)
• Ishihara A, Bartlett JS, Bertone AL Inflammation and immune response of intra-articular serotype 2 adeno-associated virus or adenovirus vectors in a large animal model. Arthritis, 2012-01-11;2012(0):735472. 2012-01-11 [PMID: 22288012] (Equine)

FAQs for IL-1ra/IL-1F3/IL1RN (DY2466). (Showing 1 - 1 of 1 FAQs).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.