Epsin-2 Antibody (OTI1G3) - Azide and BSA Free

Western Blot: Epsin-2 Antibody (OTI1G3) - Azide and BSA Free [NBP2-71701] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Epsin-2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Epsin-2.

Immunocytochemistry/Immunofluorescence: Epsin-2 Antibody (OTI1G3) - Azide and BSA Free [NBP2-71701] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Epsin-2.

Immunohistochemistry: Epsin-2 Antibody (OTI1G3) - Azide and BSA Free [NBP2-71701] - Staining of paraffin-embedded Human endometrium tissue using anti-Epsin-2 mouse monoclonal antibody.

Western Blot: Epsin-2 Antibody (OTI1G3) - Azide and BSA Free [NBP2-71701] - Analysis of extracts (35ug) from 9 different cell lines by using anti-Epsin-2 monoclonal antibody.

Immunohistochemistry: Epsin-2 Antibody (OTI1G3) - Azide and BSA Free [NBP2-71701] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-Epsin-2 mouse monoclonal antibody.

Immunohistochemistry: Epsin-2 Antibody (OTI1G3) - Azide and BSA Free [NBP2-71701] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-Epsin-2 mouse monoclonal antibody.

Immunohistochemistry: Epsin-2 Antibody (OTI1G3) - Azide and BSA Free [NBP2-71701] - Staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-Epsin-2 mouse monoclonal antibody.

Immunohistochemistry: Epsin-2 Antibody (OTI1G3) - Azide and BSA Free [NBP2-71701] - Staining of paraffin-embedded Human breast tissue using anti-Epsin-2 mouse monoclonal antibody.

Immunohistochemistry: Epsin-2 Antibody (OTI1G3) - Azide and BSA Free [NBP2-71701] - Staining of paraffin-embedded Human tonsil using anti-Epsin-2 mouse monoclonal antibody.

CyTOF-ready: Epsin-2 Antibody (OTI1G3) - Azide and BSA Free [NBP2-71701] - Analysis of Jurkat cells, using anti-EPN2 antibody , (Red), compared to a nonspecific negative control antibody, (Blue).

• Reactivity: Hu, Mu, Rt, Pm, Pm
• Applications: WB, Flow, ICC/IF, IHC, CyTOF-ready
• Clone: OTI1G3
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

Epsin-2 Antibody (OTI1G3) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse Epsin-2 Antibody (OTI1G3) - Azide and BSA Free (NBP2-01775) is a monoclonal antibody validated for use in IHC, WB, Flow and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Full length human recombinant protein of human EPN2(NP_055779) produced in HEK293T cell.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: EPN2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 1:100
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin
  • Western Blot 1:500-2000
• Theoretical MW: 68.3 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from PBS (pH 7.3) with 8% Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Immunogen affinity purified
• Reconstitution Instructions: we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Alternate Names for Epsin-2 Antibody (OTI1G3) - Azide and BSA Free

• Eps15 binding protein
• EPS-15-interacting protein 2
• epsin 2
• epsin-2
• KIAA1065EHB21

Background

Elucidation of the mechanism by which receptor tyrosine kinases (RTKs) modulate cellular physiology in response to stimuli is critical to the understanding of growth regulation. Miscues in RTK signaling pathways can result in cellular transformation and ultimately in cancer. Two novel EGF receptor substrates designated EGF-receptor pathway substrates 8 and 15, or Eps8 and Eps15, have been described. Epsin is a 90 kDa binding partner to Eps15. Both epsin and Eps15 have an ubiquitous tissue distribution but are concentrated in presynaptic nerve terminals specialized for the Clathrin-mediated endocytosis of synaptic vesicles. Disruption of epsin function blocks Clathrinmediated endocytosis. Epsin along with its binding partner Eps15 is proposed to be involved in the assistance of Clathrin coat rearrangement during Clathrin coated pit invagination. Epsin 2 and epsin 2a are also associated with Clathrin-mediated endocytosis and are enriched in the brain in the peri-Golgi region.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: EPN2