Epsin-2 Antibody (OTI1G3)

Western Blot: Epsin-2 Antibody (OTI1G3) [NBP2-01775] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Epsin-2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Epsin-2.

Immunocytochemistry/Immunofluorescence: Epsin-2 Antibody (OTI1G3) [NBP2-01775] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Epsin-2.

Immunohistochemistry-Paraffin: Epsin-2 Antibody (OTI1G3) [NBP2-01775] - Staining of paraffin-embedded Human tonsil using anti-Epsin-2 mouse monoclonal antibody.

Flow Cytometry: Epsin-2 Antibody (OTI1G3) [NBP2-01775] - Analysis of Jurkat cells, using anti-EPN2 antibody , (Red), compared to a nonspecific negative control antibody, (Blue).

Western Blot: Epsin-2 Antibody (OTI1G3) [NBP2-01775] - Analysis of extracts (35ug) from 9 different cell lines by using anti-Epsin-2 monoclonal antibody.

Immunohistochemistry-Paraffin: Epsin-2 Antibody (OTI1G3) [NBP2-01775] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-Epsin-2 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: Epsin-2 Antibody (OTI1G3) [NBP2-01775] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-Epsin-2 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: Epsin-2 Antibody (OTI1G3) [NBP2-01775] - Staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-Epsin-2 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: Epsin-2 Antibody (OTI1G3) [NBP2-01775] - Staining of paraffin-embedded Human breast tissue using anti-Epsin-2 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: Epsin-2 Antibody (OTI1G3) [NBP2-01775] - Staining of paraffin-embedded Human endometrium tissue using anti-Epsin-2 mouse monoclonal antibody.

• Reactivity: Hu, Mu, Rt, Pm, Pm
• Applications: WB, Flow, ICC/IF, IHC
• Clone: OTI1G3
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 0.73 mg/ml

Epsin-2 Antibody (OTI1G3) Summary

• Immunogen: Full length human recombinant protein of human EPN2(NP_055779) produced in HEK293T cell.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: EPN2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:100
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin 1:150
  • Western Blot 1:500-2000
• Theoretical MW: 68.3 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 1.0% BSA and 50% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 0.73 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Epsin-2 Antibody (OTI1G3)

• Eps15 binding protein
• EPS-15-interacting protein 2
• epsin 2
• epsin-2
• KIAA1065EHB21

Background

This gene encodes a protein which interacts with clathrin and adaptor-related protein complex 2, alpha 1 subunit. The protein is found in a brain-derived clathrin-coated vesicle fraction and localizes to the peri-Golgi region and the cell periphery. The protein is thought to be involved in clathrin-mediated endocytosis. Alternate splicing of this gene results in multiple transcript variants encoding different isoforms. [provided by RefSeq].

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: EPN2
• Entrez:
  • 22905(Human)