EPRS Antibody - BSA Free

Western Blot: EPRS Antibody [NBP3-12962] - EPRS antibody detects EPRS protein by western blot analysis. Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with EPRS antibody (NBP3-12962) diluted at a dilution of 1:1000.

Immunohistochemistry-Paraffin: EPRS Antibody [NBP3-12962] - EPRS antibody detects EPRS protein at cytoplasm in rat brain by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. EPRS antibody (NBP3-12962) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Immunohistochemistry-Paraffin: EPRS Antibody [NBP3-12962] - EPRS antibody detects EPRS protein at cytoplasm in mouse intestine by immunohistochemical analysis. Sample: Paraffin-embedded mouse intestine. EPRS antibody (NBP3-12962) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Immunoprecipitation: EPRS Antibody [NBP3-12962] - Immunoprecipitation of EPRS protein from 293T whole cell extracts using 5 ug of EPRS antibody (NBP3-12962). Western blot analysis was performed using EPRS antibody (NBP3-12962) diluted at 1:2500. EasyBlot anti-Rabbit IgG was used as a secondary reagent.

• Reactivity: Hu, Mu, Rt, Dr, Bv
• Applications: WB, IHC, IP, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

EPRS Antibody - BSA Free Summary

• Description: Centrifuge briefly prior to opening.
• Immunogen: Recombinant protein encompassing a sequence within the C-terminus region of human EPRS. The exact sequence is proprietary.
• Predicted Species: Bovine (90%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: EPRS1
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry Whole-Mount
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Immunoprecipitation
  • Western Blot

Reactivity Notes

Mouse (86%), Rat (83%), Chicken (84%)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7), 20% Glycerol
• Preservative: 0.025% Proclin 300
• Purity: Antigen Affinity-purified

Alternate Names for EPRS Antibody - BSA Free

• bifunctional aminoacyl-tRNA synthetase
• Cell proliferation-inducing gene 32 protein
• DKFZp313B047
• EARS
• EC 6.1.1
• EC 6.1.1.15
• EC 6.1.1.18
• GLNS
• GLUPRORS
• glutamate tRNA ligase
• glutaminyl-tRNA synthetase
• glutamyl-prolyl-tRNA synthetase
• PARSproliferation-inducing gene 32 protein
• proliferation-inducing protein 32
• proline tRNA ligase
• proline-tRNA ligase
• prolyl-tRNA synthetase
• QARS
• QPRS

Background

Glutamyl Prolyl tRNA synthetase (EPRS) is a component of ARS multi complex. EPRS is released and phosphorylated by IFN stimulation and forms a complex with GAIT.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: EPRS1