epithelial Sodium Channel gamma Antibody

Western Blot: epithelial Sodium Channel gamma Antibody [NBP2-76964] - Western blot analysis of SCNN1G on A431 cell lysate using anti-SCNN1G antibody at 1/1,000 dilution.

Immunocytochemistry/Immunofluorescence: epithelial Sodium Channel gamma Antibody [NBP2-76964] - ICC staining SCNN1G in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry: epithelial Sodium Channel gamma Antibody [NBP2-76964] - Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-SCNN1G antibody. Counter stained with hematoxylin.

Flow Cytometry: epithelial Sodium Channel gamma Antibody [NBP2-76964] - Flow cytometric analysis of 293T cells with SCNN1G antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.

Immunocytochemistry/Immunofluorescence: epithelial Sodium Channel gamma Antibody [NBP2-76964] - ICC staining SCNN1G in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry: epithelial Sodium Channel gamma Antibody [NBP2-76964] - Immunohistochemical analysis of paraffin-embedded rat kidney tissue using anti-SCNN1G antibody. Counter stained with hematoxylin.

Product Discontinued

epithelial Sodium Channel gamma Antibody Summary

• Immunogen: Recombinant protein within Human epithelial Sodium Channel gamma aa 160-342 / 649. (SwissProt: P51170 Human; SwissProt: Q9WU39 Mouse; SwissProt: P37091 Rat)
• Localization: Plasma membrane.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SCNN1G
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:500-1:2000
• Theoretical MW: 74 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH7.4), 0.2% BSA, 50% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for epithelial Sodium Channel gamma Antibody

• amiloride-sensitive epithelial sodium channel gamma subunit
• amiloride-sensitive sodium channel gamma-subunit
• BESC3
• ENaC gamma subunit
• ENaCG
• ENaCgamma
• Epithelial Na(+) channel subunit gamma
• gamma-ENaC
• gamma-NaCH
• Nonvoltage-gated sodium channel 1 subunit gamma
• PHA1
• SCNEGamiloride-sensitive sodium channel subunit gamma
• sodium channel, nonvoltage-gated 1, gamma

Background

Nonvoltage-gated, amiloride-sensitive, sodium channels control fluid and electrolyte transport across epithelia in many organs. These channels are heteromeric complexes consisting of 3 subunits: alpha, beta, and gamma. This gene encodes the gamma subunit, and mutations in this gene have been associated with Liddle syndrome. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SCNN1G