epithelial Sodium Channel beta [p Ser633] Antibody

Product Discontinued

epithelial Sodium Channel beta [p Ser633] Antibody Summary

• Immunogen: A synthetic peptide to phospho S633 of rat amiloride-sensitive sodium channel subunit beta (SCNN1B, SCNEB) conjugated to an immunogenic carrier protein was used as the immunogen. The antigen is homologous with the corresponding sequence in mouse.
• Modification: p Ser633
• Specificity: Specific for SCNN1B.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: SCNN1B
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
• Application Notes: Note that this antibody may also recognise the unphosphorylated form.

Reactivity Notes

Rat, mouse. Other species not yet tested.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Whole antisera
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Unpurified
• Reconstitution Instructions: Reconstitute in 100 ul of sterile water. Centrifuge to remove any insoluble material. Glycerol may be added (1:1) for additional stability.

Alternate Names for epithelial Sodium Channel beta [p Ser633] Antibody

• amiloride-sensitive sodium channel subunit beta 1
• amiloride-sensitive sodium channel subunit beta
• BESC1
• Beta-ENaC
• Beta-NaCH
• ENaCB
• ENaCbeta
• Epithelial Na(+) channel subunit beta
• epithelial Sodium Channel beta
• epithelial sodium channel beta-2 subunit
• epithelial sodium channel beta-3 subunit
• nasal epithelial sodium channel beta subunit
• Nonvoltage-gated sodium channel 1 subunit beta
• SCNEB
• SCNN1B
• sodium channel, nonvoltage-gated 1, beta

Background

SCNN1B is a subunit of the epithelial sodium channel, ENaC. ENac has high sodium selectivity, low conductance, and amiloride sensitivity. The functional channel of ENaC is composed of at least 3 subunits, alpha (SCNN1A), beta (SCNN1B), and gamma (SCNN1G). The 3 subunits show sequence similarities to one another, indicating descent from a common ancestral gene. Each encodes a protein containing 2 transmembrane domains, with intracellular amino and carboxyl termini. ENaC in the kidney, lung and colon plays an essential role in trans- epithelial sodium and fluid balance. ENaC also mediates aldosterone-dependent sodium re-uptake in the distal nephron of the kidney, thus regulating blood pressure. Mutations in beta or gamma subunit of the epithelial sodium channel (ENaC) have been found to cause a hereditary form of human hypertension, Liddle syndrome.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SCNN1B
• Entrez:
  • 6338(Human)