epithelial Sodium Channel beta Antibody (14E10) [Alexa Fluor™ Plus 555]

• Reactivity: Mu, Rt
• Applications: WB, IHC
• Clone: 14E10
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor Plus 555

epithelial Sodium Channel beta Antibody (14E10) [Alexa Fluor™ Plus 555] Summary

• Immunogen: Synthetic peptide from the N-terminal of Rat ENaC alpha (aa. 46-68)
• Localization: Apical Cell Membrane
• Specificity: Detects 85kDa.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: SCNN1B
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Western Blot
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Protein G purified

Notes

This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for epithelial Sodium Channel beta Antibody (14E10) [Alexa Fluor™ Plus 555]

• amiloride-sensitive sodium channel subunit beta 1
• amiloride-sensitive sodium channel subunit beta
• BESC1
• Beta-ENaC
• Beta-NaCH
• ENaCB
• ENaCbeta
• Epithelial Na(+) channel subunit beta
• epithelial Sodium Channel beta
• epithelial sodium channel beta-2 subunit
• epithelial sodium channel beta-3 subunit
• nasal epithelial sodium channel beta subunit
• Nonvoltage-gated sodium channel 1 subunit beta
• SCNEB
• SCNN1B
• sodium channel, nonvoltage-gated 1, beta

Background

SCNN1B is a subunit of the epithelial sodium channel, ENaC. ENac has high sodium selectivity, low conductance, and amiloride sensitivity. The functional channel of ENaC is composed of at least 3 subunits, alpha (SCNN1A), beta (SCNN1B), and gamma (SCNN1G). The 3 subunits show sequence similarities to one another, indicating descent from a common ancestral gene. Each encodes a protein containing 2 transmembrane domains, with intracellular amino and carboxyl termini. ENaC in the kidney, lung and colon plays an essential role in trans- epithelial sodium and fluid balance. ENaC also mediates aldosterone-dependent sodium re-uptake in the distal nephron of the kidney, thus regulating blood pressure. Mutations in beta or gamma subunit of the epithelial sodium channel (ENaC) have been found to cause a hereditary form of human hypertension, Liddle syndrome.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SCNN1B