EPHX1 Antibody - BSA Free

Immunocytochemistry/Immunofluorescence: EPHX1 Antibody [NBP1-33011] - Analysis of methanol-fixed HeLa, using EPHX1 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with an alpha Tubulin antibody (Red) at 1:2000.

Immunohistochemistry-Paraffin: EPHX1 Antibody [NBP1-33011] - Mouse liver. EPHX1 antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

Immunohistochemistry-Paraffin: EPHX1 Antibody [NBP1-33011] - SG xenograft, using Epoxide hydrolase 1 antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with EPHX1 antibody (NBP1-33011) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with EPHX1 antibody (NBP1-33011) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

• Reactivity: Hu, Mu
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

EPHX1 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit EPHX1 Antibody - BSA Free (NBP1-33011) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. Anti-EPHX1 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human EPHX1. The exact sequence is proprietary.
• Localization: Microsome membrane, Single-pass membrane protein, Endoplasmic reticulum membrane, Single-pass membrane protein
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: EPHX1
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 53 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS, 20% Glycerol
• Preservative: 0.025% Proclin 300
• Purity: Antigen Affinity-purified

Alternate Names for EPHX1 Antibody - BSA Free

• EC 3.3.2.9
• EPHX
• EPHX1
• EPOX
• Epoxide Hydratase
• Epoxide Hydrolase 1
• epoxide hydrolase 1, microsomal (xenobiotic)
• HYL1
• MEH
• MEH8
• Microsomal Epoxide Hydrolase

Background

Epoxide hydrolase is a critical biotransformation enzyme that converts epoxides from the degradation of aromatic compounds to trans-dihydrodiols which can be conjugated and excreted from the body. Epoxide hydrolase functions in both the activation and detoxification of epoxides. Mutations in this gene cause preeclampsia, epoxide hydrolase deficiency or increased epoxide hydrolase activity. Alternatively spliced transcript variants encoding the same protein have been found for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for EPHX1 Antibody (NBP1-33011)(1)

Publication using NBP1-33011

• Gautheron J, Morisseau C, Chung WK Et al. EPHX1 mutations cause a lipoatrophic diabetes syndrome due to impaired epoxide hydrolysis and increased cellular senescence eLife 2021-08-03 [PMID: 34342583] (WB, ICC/IF, Human)

Bioinformatics

• Gene Symbol: EPHX1