Ephrin-A1 Antibody

Western Blot: Ephrin-A1 Antibody [NBP1-52103] - (0.01ug/ml) staining of Mouse Thymus lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

• Reactivity: Mu, Rt, Hu, Po, Ca
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

Ephrin-A1 Antibody Summary

• Description: Novus Biologicals Goat Ephrin-A1 Antibody (NBP1-52103) is a polyclonal antibody validated for use in WB and ELISA. Anti-Ephrin-A1 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence C-KVTVSGKITHSP corresponding to internal region according to NP_004419.2.
• Epitope: C-KVTVSGKITHSP
• Specificity: This antibody is expected to recognize isoform a (NP_004419.2) only.
• Predicted Species: Human (100%), Porcine (100%), Canine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: EFNA1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:16000
  • Western Blot 0.01 - 0.03 ug/ml
• Application Notes: WB: Approx. 26 kDa band observed in mouse thymus and rat lung lysates (calculated MW of 23.8 kDa band according to mouse NP_034237.3 and rat NP_446051.2).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Ephrin-A1 Antibody

• B61
• ECKLG
• EFL1
• EFL-1
• EFNA1
• EPH-related receptor tyrosine kinase ligand 1
• EphrinA1
• Ephrin-A1
• EPLG1TNF alpha-induced protein 4
• Immediate early response protein B61
• LERK1
• LERK1LERK-1
• ligand of eph-related kinase 1
• TNFAIP4B61
• Tumor necrosis factor alpha-induced protein 4
• tumor necrosis factor, alpha-induced protein 4

Background

This gene encodes a member of the ephrin (EPH) family. The ephrins and EPH-related receptors comprise the largest subfamily of receptor protein-tyrosine kinases and have been implicated in mediating developmental events, especially in the nervous system and in erythropoiesis. Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. This gene encodes an EFNA class ephrin which binds to the EPHA2, EPHA4, EPHA5, EPHA6, and EPHA7 receptors. Two transcript variants that encode different isoforms were identified through sequence analysis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Ephrin-A1 Antibody (NBP1-52103)(1)

Publication using NBP1-52103

• Zhang W, Zeng X, Briggs KJ et al. A potential tumor suppressor role for Hic1 in breast cancer through transcriptional repression of ephrin-A1. Oncogene 2010-04-01 [PMID: 20154726]

Bioinformatics

• Gene Symbol: EFNA1
• Entrez:
  • 1942(Human)
  • 13636(Mouse)
  • 94268(Rat)
• Uniprot:
  • NP_004419.2(Human)