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Ephrin-A1 Antibody (93036)

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Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB
Clone
93036
Clonality
Monoclonal
Host
Rat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Formulation Size Price

Ephrin-A1 Antibody (93036) Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Ephrin-A1
Ala18-Ser182 (predicted)
Accession # P52793
Specificity
Detects mouse Ephrin-A1 in direct ELISAs and Western blots. In direct ELISAs, approximately 25% cross-reactivity with recombinant mouse (rm) Ephrin-A2 is observed, and no cross-reactivity with recombinant human Ephrin-A3, -B3, rmEphrin-A4, -A5, -B1, or -B2 is observed.
Source
N/A
Isotype
IgG2b
Clonality
Monoclonal
Host
Rat
Gene
EFNA1
Purity
Protein A or G purified from hybridoma culture supernatant
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Protein A or G purified from hybridoma culture supernatant
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Ephrin-A1 Antibody (93036)

  • B61
  • ECKLG
  • EFL1
  • EFL-1
  • EFNA1
  • EPH-related receptor tyrosine kinase ligand 1
  • EphrinA1
  • Ephrin-A1
  • EPLG1TNF alpha-induced protein 4
  • Immediate early response protein B61
  • LERK1
  • LERK1LERK-1
  • ligand of eph-related kinase 1
  • TNFAIP4B61
  • Tumor necrosis factor alpha-induced protein 4
  • tumor necrosis factor, alpha-induced protein 4

Background

Ephrin-A1, also known as B61, LERK-1, and EFL-1, (1) is a member of the Ephrin ligand family which binds members of the Eph receptor family. All ligands share a conserved extracellular sequence, which most likely corresponds to the receptor binding domain. This conserved sequence consists of approximately 125 amino acids and includes four invariant cysteines. The A-class ligands have a GPI anchor following the conserved sequence. Ephrin-A1 binds EphA1, EphA2, EphA3, EphA4, EphA5, EphA6, EphA7, and EphB1 (2, 3). The extracellular domains of human and mouse Ephrin-A1 share 85% amino acid identity. Only membrane‑bound or Fc‑clustered ligands are capable of activating the receptor in vitro. While soluble monomeric ligands bind the receptor, they do not induce receptor autophosphorylation and activation (2). In vivo, the ligands and receptors display reciprocal expression (3). Nearly all receptors and ligands are expressed in developing and adult neural tissue (3). The Eph/Ephrin families also appear to play a role in angiogenesis (3).

  1. Unified nomenclature for Eph family receptors and their ligands, the ephrins. Eph Nomenclature Committee [letter]. Cell (1997) 90:403.
  2. Flanagan, J.G. and P. Vanderhaegen, The ephrins and Eph receptors in neural development. Annu. Rev. Neurosci. (1998) 21:309.
  3. Pasquale, E.B., The Eph family of receptors. Curr. Opin. Cell. Biol. (1997) 9:608.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol EFNA1
Uniprot