EphB1 Antibody [Janelia Fluor® 646]

• Reactivity: Rt
• Applications: WB, Flow, IHC, CyTOF-ready
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Janelia Fluor 646

EphB1 Antibody [Janelia Fluor® 646] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant rat EphB1
  Met18-Gln538
  Accession # P09759
• Specificity: Detects rat EphB1 in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant mouse (rm) EphA3, rmEphA4, rmEphB2, rmEphB3, rmEphB4, and rmEphB6 is observed, less than 1% cross-reactivity with recombinant human (rh) EphA1, rhEphA5, rmEphA7, rmEphA8, and recombinant rat EphA5 is observed.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: EPHB1
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry
  • Immunohistochemistry
  • Western Blot
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Antigen Affinity-purified

Notes

Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

Alternate Names for EphB1 Antibody [Janelia Fluor® 646]

• Cek6
• EC 2.7.10
• EC 2.7.10.1
• EK6
• Elk
• EPH receptor B1
• eph tyrosine kinase 2
• EphB1
• EPH-like kinase 6
• ephrin type-B receptor 1
• EPHT2FLJ37986
• Hek6
• Net
• NETHek6
• soluble EPHB1 variant 1
• Tyrosine-protein kinase receptor EPH-2

Background

EphrinB proteins are thought to play key roles in cellular functions as diverse as neuronal migration and blood vessel development. EphrinB molecules expressed at the membrane surface bind to the EphB family receptors on target cells during cell-to cell contact. This interaction leads to cell signaling in the target cell but also generates a reverse signal in the cell expressing EphrinB on its surface. This reverse signaling event is thought to be critical for vessel maturation and neuronal development. Importantly, tyrosine phosphorylation of EphrinB is thought to be a critical component of this reverse signaling event. Recent work suggests that phosphorylation of a specific EphrinB residue (Tyr298) plays a key role in EphrinB signaling. (3, 5)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: EPHB1