EphA6 Antibody (3D5B10)

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Western Blot: EphA6 Antibody (3D5B10) [NBP1-51559] - Western blot analysis using EphA6 mouse mAb against truncated MBP-EphA6 recombinant protein (1) and truncated GST-EphA6(aa695-795) recombinant protein (2).

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA
Clone
3D5B10
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

EphA6 Antibody (3D5B10) Summary

Immunogen
Purified recombinant fragment of EPH Receptor A6 (3D5B10) expressed in E. Coli.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
EPHA6
Purity
Unpurified
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Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:2000
  • ELISA 1:10000

Reactivity Notes

Human

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Ascites
Preservative
0.03% Sodium Azide
Purity
Unpurified

Alternate Names for EphA6 Antibody (3D5B10)

  • DKFZp434C1418
  • EC 2.7.10
  • EC 2.7.10.1
  • Ehk2
  • EHK-2
  • EPA6
  • EPH homology kinase 2
  • EPH receptor A6
  • EphA6
  • ephrin receptor EphA6
  • ephrin type-A receptor 6
  • FLJ35246
  • Hek12
  • PRO57066

Background

EphA6, also known as Ehk2 and Hek12, is a member of the Eph receptor family, which binds members of the Ephrin ligand family. Two classes of receptors exist, designated A and B, that have an extracellular domain made up of a globular domain, a cysteine-rich domain, and two fibronectin type III domains, followed by the transmembrane region and cytoplasmic region. The cytoplasmic region is a juxtamembrane region with two tyrosines, the major autophosphorylation sites, along with a kinase domain, and a conserved sterile alpha motif (SAM) in the C-terminus, the latter including one conserved tyrosine.The extracellular domains of mouse and rat EphA6 share approximately 99% amino acid identity. The calculated molecular mass of the reduced mouse EphA6/Fc monomer is approximately 86 kDa, but as a result of glycosylation, recombinant EphA6/Fc migrates as an approximately 100 kDa protein under reducing conditions in SDS-PAGE. Ligand recognition and binding leads to kinase activation of the intrinsic kinase activity. EphA6 binds to Ephrin A1, Ephrin A2, Ephrin A3, Ephrin A4 and Ephrin A5. Only membrane-bound or Fc-clustered ligands have been shown to activate the receptor in vitro. Soluble monomeric ligands bind the receptor, but do not induce receptor autophosphorylation and activation. The ephrin ligands and Eph receptors display reciprocal expression in vivo. Developing and adult neural tissue express nearly all of the Eph receptors and ephrin ligands. Ephs and ephrins play a significant role in angiogenesis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

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Bioinformatics

Gene Symbol EPHA6
Entrez