EPCR Antibody (M2) - Azide and BSA Free

Immunocytochemistry/Immunofluorescence: EPCR Antibody (M2) [H00010544-M03] - Analysis of monoclonal antibody to PROCR on HeLa cell. Antibody concentration 10 ug/ml

Immunohistochemistry-Paraffin: EPCR Antibody (M2) [H00010544-M03] - Analysis of monoclonal antibody to PROCR on formalin-fixed paraffin-embedded human malignant lymphoma, diffuse large B tissue. Antibody concentration 3 ug/ml

Sandwich ELISA: EPCR Antibody (M2) [H00010544-M03] - Detection limit for recombinant GST tagged PROCR is 3 ng/ml as a capture antibody.

• Reactivity: Hu, Mu
• Applications: WB, ELISA, ICC/IF, IHC
• Clone: M2
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

EPCR Antibody (M2) - Azide and BSA Free Summary

• Immunogen: PROCR (AAH14451.1, 1 a.a. ~ 238 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MLTTLLPILLLSGWAFCSQDASDGLQRLHMLQISYFRDPYHVWYQGNASLGGHLTHVLEGPDTNTTIIQLQPLQEPESWARTQSGLQSYLLQFHGLVRLVHQERTLAFPLTIRCFLGCELPPEGSRAHVFFEVAVNGSSFVSFRPERALWQADTQVTSGVVTFTLQQLNAYNRTRYELREFLEDTCVQYVQKHISAENTKGSQTSRSYTSLVLGVLVGGFIIAGVAVGIFLCTGGRRC
• Specificity: PROCR - protein C receptor, endothelial (EPCR)
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: PROCR
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Sandwich ELISA
  • Western Blot 1:500
• Application Notes: Antibody reactive against recombinant protein for Western Blot. Has also been used for immunofluoresence, immunohistochemistry (paraffin), and ELISA.

Reactivity Notes

Other species not tested.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for EPCR Antibody (M2) - Azide and BSA Free

• APC receptor
• CCCA
• CCD41
• CCD41centrosome-associated protein
• CD201 antigen
• CD201
• centrocyclin
• Endothelial cell protein C receptor
• endothelial protein C receptor
• EPCR
• EPCRMGC23024
• PROCR
• protein C receptor, endothelial

Background

The protein encoded by this gene is a receptor for activated protein C, a serine protease activated by and involved in the blood coagulation pathway. The encoded protein is an N-glycosylated type I membrane protein that enhances the activation of protein C. Mutations in this gene have been associated with venous thromboembolism and myocardial infarction, as well as with late fetal loss during pregnancy.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for EPCR Antibody (H00010544-M03)(7)

Publications using H00010544-M03

• Kwak S, Han MS, Bae JS. Aspalathin and nothofagin from rooibos (Aspalathus linearis) inhibit endothelial protein C receptor shedding in vitro and in vivo. Fitoterapia 2014-12-12 [PMID: 25510322]
• Ku SK, Zhou W, Lee W et al. Anti-inflammatory effects of hyperoside in human endothelial cells and in mice. Inflammation 2014-08-06 [PMID: 25097077]
• Ku SK, Han MS, Bae JS. Sulforaphane inhibits endothelial protein C receptor shedding in vitro and in vivo. Vascul Pharmacol. 2014-07-09 [PMID: 25016099]
• Ku SK, Lee W, Yoo H et al. Inhibitory effects of epi-sesamin on endothelial protein C receptor shedding in vitro and in vivo. Inflamm Res. 2013-07-25 [PMID: 23884236]
• Ku SK, Yang EJ, Song KS, Bae JS. Rosmarinic acid down-regulates endothelial protein C receptor shedding in vitro and in vivo. Food Chem Toxicol. 2013-06-14 [PMID: 23774263]
• Ku SK, Han MS, Bae JS. Down-regulation of endothelial protein C receptor shedding by persicarin and isorhamnetin-3-O-galactoside. Thromb Res. 2013-05-30 [PMID: 23726966]
• Lee W, Ku SK, Bae JS. Emodin-6-O-b-D-glucoside down-regulates endothelial protein C receptor shedding. Arch Pharm Res. 2013-04-16 [PMID: 23588681]

Bioinformatics

• Gene Symbol: PROCR
• Entrez:
  • 10544(Human)
• OMIM:
  • 600646(Human)
• Uniprot:
  • AAH14451(Human)