Human ENPP-7/Alk-SMase ELISA Kit (Chemiluminescence)

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ELISA: Human ENPP-7/Alk-SMase ELISA Kit (Chemiluminescence) [NBP2-68255] - Samples were spiked with high concentrations of Human ENPP-7/Alk-SMase and diluted with Reference Standard & Sample Diluent to produce samples ...read more
ELISA: Human ENPP-7/Alk-SMase ELISA Kit (Chemiluminescence) [NBP2-68255] - Standard Curve Reference

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA
Suitable Sample Type
Serum, plasma and other biological fluids
Standard Curve Range
78.13-5000 pg/mL
Sensitivity
46.88 pg/mL

Order Details

Human ENPP-7/Alk-SMase ELISA Kit (Chemiluminescence) Summary

Specificity
This kit recognizes Human Alk-Smase in samples. No significant cross-reactivity or interference between Human Alk-Smase and analogues was observed.
Standard Curve Range
78.13-5000 pg/mL
Sensitivity
46.88 pg/mL
Assay Type
Sandwich-CLIA
Inter-Assay
CV% < 8.78%
Intra-Assay
CV% < 8.58%
Spike Recovery
91-115%
Sample Volume
100 uL
Kit Type
ELISA Kit (Chemiluminescence)
Gene
ENPP7

Applications/Dilutions

Dilutions
  • ELISA

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Biotinylated Detection Ab Diluent
  2. Concentrated Biotinylated Detection Ab (100x)
  3. Concentrated HRP Conjugate (100x)
  4. Concentrated Wash Buffer (25x)
  5. HRP Conjugate Diluent
  6. Micro CLIA Plate (Dismountable)
  7. Plate Sealer
  8. Product Manual
  9. Reference Standard
  10. Sample Diluent
  11. Substrate Reagent A
  12. Substrate Reagent B

Alternate Names for Human ENPP-7/Alk-SMase ELISA Kit (Chemiluminescence)

  • Alkaline sphingomyelin phosphodiesterase
  • alkaline sphingomyelinase
  • Alk-SMase
  • EC 3.1.4.12
  • ectonucleotide pyrophosphatase/phosphodiesterase 7
  • ectonucleotide pyrophosphatase/phosphodiesterase family member 7
  • E-NPP 7
  • ENPP7
  • ENPP-7
  • Intestinal alkaline sphingomyelinase
  • MGC50179
  • NPP7
  • NPP-7

Background

Converts sphingomyelin to ceramide. Also has phospholipase C activity toward palmitoyl lyso-phosphocholine.Does not appear to have nucleotide pyrophosphatase activity

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Bioinformatics

Gene Symbol ENPP7