ENPP-7/Alk-SMase Antibody [Alexa Fluor™ Plus 680]

• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Alexa Fluor Plus 680

ENPP-7/Alk-SMase Antibody [Alexa Fluor™ Plus 680] Summary

• Specificity: Detects mouse ENPP‑7/Alk‑SMase in direct ELISAs and Western blots. In direct ELISAs, approximately 50% cross‑reactivity with recombinant human ENPP-7 is observed.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Background

ENPP7 (ectonucleotide pyrophosphatase/phosphodiesterase 7), also known as alkaline sphingomyelinase (Alk-SMase) is expressed in the intestines and in bile (1). It shares 30-36% homology with the members of the nucleotide pyrophosphatase/phosphodiesterese (NPP) family while sharing no similarities with neutral or acid SMase (2). Its main function is the digestion of dietary sphingomyelin by hydrolyzing sphingomyelin into ceramide and phosphorylcholine. ENPP7 is reported to hydrolyse and inactivate platelet-activating factor (PAF) by a phospholipase C-type activity (3). Studies show a decrease in ENPP7 activity in human colorectal adenocarcinomas and human colorectal carcinomas, which indicate a potential role of ENPP7 in human colon cancer (4, 5).

1. Duan, R-D. et al. (2006) Biochim. Biophys. 1761:281.
2. Duan, R-D. et al. (2003) J. Biol. Chem. 278:38528.
3. Wu, J. et al. (2006) Biochem J. 394:299.
4. Hertervig, E. et al. (1996) Cancer. 79:448.
5. Hertervig, E. et al. (1999) Br. J. Cancer. 81:232.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.