ENPP-7/Alk-SMase Antibody (560514) [Alexa Fluor® 350]

• Reactivity: Hu
• Applications: WB
• Clone: 560514
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 350

ENPP-7/Alk-SMase Antibody (560514) [Alexa Fluor® 350] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human ENPP‑7/Alk‑SMase
  Pro23-Ser439
  Accession # Q6UWV6
• Specificity: Detects human ENPP‑7/Alk‑SMase in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human (rh) ENPP-2, rhENPP-5, recombinant mouse ENPP-7 is observed.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for ENPP-7/Alk-SMase Antibody (560514) [Alexa Fluor® 350]

• Alkaline sphingomyelin phosphodiesterase
• alkaline sphingomyelinase
• Alk-SMase
• EC 3.1.4.12
• ectonucleotide pyrophosphatase/phosphodiesterase 7
• ectonucleotide pyrophosphatase/phosphodiesterase family member 7
• E-NPP 7
• ENPP7
• ENPP-7
• Intestinal alkaline sphingomyelinase
• MGC50179
• NPP7
• NPP-7

Background

ENPP-7 (ectonucleotide pyrophosphatase/phosphodiesterase 7), also known as alkaline sphingomyelinase (Alk-SMase) is a 60 kDa GPI-linked membrane glycoprotein expressed in the intestines and in human bile. It shares 30%‑36% homology with the NPP family, but is unrelated to neutral or acid SMase. ENPP-7 hydrolyzes dietary sphingomyelin to form ceramide and phosphorylcholine, and may also hydrolyze and inactivate platelet-activating factor (PAF). It is down‑regulated in some human colorectal carcinomas. The 416 amino acid (aa) mature human ENPP-7 shares 80% and 82% aa identity with mouse and rat ENPP-7, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.