Enolase 2/Neuron-specific Enolase Antibody (522601.211) [Unconjugated]

Enolase 2/Neuron‑specific Enolase was detected in immersion fixed SH‑SY5Y human neuroblastoma cell line using Mouse Anti-Human Enolase 2/Neuron‑specific Enolase Monoclonal Antibody (Catalog # MAB5169) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to plasma membrane and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

• Reactivity: Hu
• Applications: IHC, IP, ICC/IF
• Clone: 522601.211
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

Enolase 2/Neuron-specific Enolase Antibody (522601.211) [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human Enolase 2/Neuron-specific Enolase
  Met1-Leu434
  Accession # P09104
• Specificity: Detects human Enolase 2/Neuron-specific Enolase in direct ELISAs.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: ENO2

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 8-25 ug/mL
  • Immunoprecipitation 25 ug/mL
  • Immunocytochemistry 8-25 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS and NaCl with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Enolase 2/Neuron-specific Enolase Antibody (522601.211) [Unconjugated]

• 2-phospho-D-glycerate hydrolyase
• 2-phospho-D-glycerate hydro-lyase
• EC 4.2.1.11
• ENO2
• enolase 2 (gamma, neuronal)
• Enolase 2
• gamma-Enolase
• Neural enolase
• neuron specific gamma enolase
• neurone-specific enolase
• Neuronspecific Enolase
• Neuron-specific enolase
• NSE

Background

Enolase 2 (2-phospho-D-glycerate hydrolyase; also Neuron-specific Enolase, NSE, neural enolase and gamma -enolase) is a 46 kDa member of the Enolase family of enzymes. It is expressed in developing neurons and glia, is known to catalyze the generation of phosphoenolpyruvate, and is suggested to possess neurotrophic activity for neurons, likely through an extracellular mechanism. Human Enolase 2 is 434 amino acids (aa) in length. The enzymatic site spans most of the length of the molecule. Enolase 2 exists as both a noncovalently-linked homodimer, or heterodimer with alpha -enolase. Full-length human Enolase 2 shares 99% aa identity with both mouse and canine Enolase 2. It shares 83% aa identity with human enolases 1 and 3.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ENO2
• Uniprot:
  • P09104()