EMR1 Antibody

Images

 
Immunohistochemistry-Paraffin: EMR1 Antibody [NBP2-31090] - IHC of human bone marrow. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

EMR1 Antibody Summary

Immunogen
EMR1 / F4/80 antibody was raised against synthetic 19 amino acid peptide from N-terminal extracellular domain of human EMR1.
Epitope
N-Terminus
Specificity
Human EMR1. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
EMR1
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • ELISA 1:100-1:2000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 18-36 ug/ml

Reactivity Notes

Expected cross-reactivity based on sequence identity: Gorilla (89%).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.1% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for EMR1 Antibody

  • ADGRE1
  • egf-like module containing, mucin-like, hormone receptor-like 1
  • egf-like module containing, mucin-like, hormone receptor-like sequence 1
  • EGF-like module receptor 1
  • EGF-like module-containing mucin-like hormone receptor-like 1
  • EMR1 hormone receptor
  • EMR1
  • TM7LN3

Background

EGF-Like Module Containing Mucin-Like Hormone Receptor-Like Sequence 1 (EMR1) is characterized as an Orphan-B GPCR with an unknown ligand. The C-terminus contains seven hydrophobic segments, characteristic of a G protein-coupled receptor. The N-terminus of EMR1 has 6 EGF-like modules, separated from the transmembrane segments by a serine/threonine-rich domain, a feature reminiscent of mucin-like, single-span, integral membrane glycoproteins with adhesive properties. F4/80 is a monoclonal antibody that recognizes a murine macrophage- restricted cell surface glycoprotein whose amino acid sequence has been determined to be the EMR1 gene. Therefore, the data from this antibody can be used to derive gene expression of mouse EMR1. EMR1 has been reported to be expressed in human peripheral blood mononuclear cells, monocytes, and hematopoietic and solid tumor-derived cell lines. ESTs have been isolated from human normal spleen and mixed liver/spleen libraries and from human kidney, colon, brain, and bone marrow cancer libraries.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol EMR1
Entrez
Uniprot