EME1 Antibody (MTA31 7H2/1) - Azide and BSA Free

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC, IP
Clone
MTA31 7H2/1
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
Azide and BSA Free
Concentration
1 mg/ml

Order Details

EME1 Antibody (MTA31 7H2/1) - Azide and BSA Free Summary

Description
1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP3-07779).

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Immunogen
His-tagged recombinant Eme1 of human origin (Uniprot: Q96AY2)
Localization
Nucleus
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
EME1
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1-2 ug/ml
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation 1-2 ug per 100-500 ug of protein 1ml of lysate
  • Western Blot 1-2 ug/ml
Application Notes
Immunohistochemistry Formalin-fixed: 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined.
Theoretical MW
65 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Buffer
10 mM PBS
Preservative
No Preservative
Concentration
1 mg/ml
Purity
Protein A or G purified

Alternate Names for EME1 Antibody (MTA31 7H2/1) - Azide and BSA Free

  • crossover junction endonuclease EME1
  • EC 3.1.22
  • EC 3.1.22.-
  • essential meiotic endonuclease 1 homolog 1 (S. pombe)
  • essential meiotic endonuclease 1 homolog 2
  • FLJ31364
  • hMMS4
  • homolog of yeast EME1 endonuclease
  • MMS4 homolog
  • MMS4
  • MMS4L
  • SLX2 structure-specific endonuclease subunit homolog A
  • SLX2A

Background

EME1 and MUS81 (MIM 606591) form an endonuclease complex that cleaves branched DNA structures, especially those arising during stalled DNA replication (Abraham et al., 2003 [PubMed 14609959]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for EME1 Antibody (NBP3-08496) (0)

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Secondary Antibodies

 

Isotype Controls

Additional EME1 Products

Array NBP3-08496

Research Areas for EME1 Antibody (NBP3-08496)

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Bioinformatics

Gene Symbol EME1