Elk-1 [p Ser389] Antibody

Images

 
Western Blot: Elk1 [p Ser389] Antibody [NBP2-54759] - Western blot analysis of lysed extracts from HT29 cells untreated or treated with Anisomycin using Elk1 [p-Ser389]
Immunohistochemistry-Paraffin: Elk1 [p Ser389] Antibody [NBP2-54759] - Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Elk1 [P-Ser389].

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

Elk-1 [p Ser389] Antibody Summary

Immunogen
Elk1 (Phospho-Ser389) antibody was raised against a peptide sequence around phosphorylation site of serine 389 (P-R-S (p) -P-A) derived from Human Elk-1.
Modification
p Ser389
Specificity
This antibody detects endogenous level of Elk-1 only when phosphorylated at serine 389.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ELK1
Purity
Epitope affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA 1 ug/ml
  • Immunohistochemistry 1:50-1:100
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Western Blot 1:500-1:1000
Theoretical MW
62 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20 degrees C. Avoid freeze/thaw cycles.
Buffer
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, and 50% glycerol.
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Epitope affinity purified

Alternate Names for Elk-1 [p Ser389] Antibody

  • Elk1
  • Elk-1
  • ELK1, member of ETS oncogene family
  • ETS domain-containing protein Elk-1
  • ETS-like gene 1

Background

The transcription factor ELK1 is a family of member of ETS oncogene family and of the ternary complex factor (TCF) subfamily,which is located on chromosome Xp11.2 and stimulates transcription. binds to purine-rich DNA sequences. Proteins of the TCF subfamily form a ternary complex by binding to the the serum response factor and the serum reponse element in the promoter of the c-fos proto-oncogene. The protein encoded by this gene is a nuclear target for the ras-raf-MAPK signaling cascade. Elk1 is phosphorylated by MAP kinase pathways at a cluster of S/T motifs at its C terminus,It appears to be a direct target of activated MAP kinase. Biochemical studies indicate that Elk1 is a good substrate for MAP kinase, the kinetics of Elk1phosphorylation and activation correlate with MAP kinase activity, and interfering mutants of MAP kinase block Elk1 activation in vivo. More recent studies have shown that Elk1 is also a target of the Stress Activated Kinase SAPK/JNK. Phosphorylation of Elk1 has also been implicated in synaptic plasticity in the adult hippocampus.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol ELK1