Elastase Antibody (CELA3B/1257)

Images

 
Immunohistochemistry-Paraffin: Elastase Antibody (CELA3B/1257) [NBP2-47645] - Formalin-fixed, paraffin-embedded Mouse Pancreas stained with CELA3B Monoclonal Antibody (CELA3B/1257).
Immunohistochemistry-Paraffin: Elastase Antibody (CELA3B/1257) [NBP2-47645] - Formalin-fixed, paraffin-embedded Human Pancreas stained with CELA3B Monoclonal Antibody (CELA3B/1257)
Immunohistochemistry-Paraffin: Elastase Antibody (CELA3B/1257) [NBP2-47645] - Formalin-fixed, paraffin-embedded Rat Pancreas stained with CELA3B Monoclonal Antibody (CELA3B/1257).
SDS-Page: Elastase Antibody (CELA3B/1257) [NBP2-47645]

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications Flow, IHC-P, PAGE, IF
Clone
CELA3B/1257
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

Elastase Antibody (CELA3B/1257) Summary

Immunogen
Recombinant human CELA3B Recombinant human CELA3B fragment of 157 amino acid residues (aa82-238)
Localization
Cytoplasmic
Marker
Pancreatic Function Marker
Specificity
This MAb recognizes a protein of ~30kDa, identified as CELA3B (Chymotrypsin like elastase family member 3B). Elastases form a subfamily of serine proteases that hydrolyze many proteins in addition to elastin. Humans have six elastase genes which encode the structurally similar proteins elastase 1, 2, 2A, 2B, 3A, and 3B. Unlike other elastases, elastase 3B has little elastolytic activity. Like most of the human elastases, elastase 3B is secreted from the pancreas as a zymogen and, like other serine proteases such as trypsin, chymotrypsin and kallikrein; it has a digestive function in the intestine. Elastase 3B preferentially cleaves proteins after alanine residues. Elastase 3B may also function in the intestinal transport and metabolism of cholesterol. Both elastase 3A and elastase 3B have been referred to as protease E and as elastase 1, and excretion of this protein in fecal material is frequently used as a measure of pancreatic function in clinical assays.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
CELA3B
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 0.5-1ug/million cells
  • Immunohistochemistry-Paraffin 0.5-1ug/ml
  • SDS-Page
  • Immunofluorescence 0.5-1ug/ml
Application Notes
Human Chromosome Location: 1p36.12
Immunohistology (Formalin-fixed) (0.5-1ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes)
Theoretical MW
17 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C.
Buffer
10mM PBS and 0.05% BSA
Preservative
0.05% Sodium Azide
Purity
Protein A or G purified

Alternate Names for Elastase Antibody (CELA3B/1257)

  • CBPP
  • cholesterol-binding pancreatic protease
  • chymotrypsin-like elastase family member 3B
  • chymotrypsin-like elastase family, member 3B
  • E1
  • EC 3.4.21
  • EC 3.4.21.70
  • EL-1
  • ELA3B
  • elastase 1
  • elastase 3B, pancreatic
  • Elastase IIIB
  • elastase-3B
  • fecal elastase 1
  • pancreatic elastase 1
  • pancreatic endopeptidase E
  • Protease E
  • proteinase E

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP1-89522
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-31302
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-89150
Species: Hu, Mu, Rt
Applications: WB, IHC, IHC-P
H00011026-M01
Species: Hu
Applications: WB, ELISA
NBP1-58268
Species: Hu
Applications: WB, IHC, IHC-P
H00008458-M06
Species: Hu, Mu, Rt
Applications: WB, ELISA, ICC/IF
NBP1-84943
Species: Hu, Mu, Rt
Applications: WB, IHC, IHC-P
H00009354-M08
Species: Hu, Mu, Rt
Applications: WB, ELISA, ICC/IF
NBP1-88927
Species: Hu
Applications: ICC/IF, IHC, IHC-P
NBP2-34260
Species: Hu, Mu, Po, Bv, Rb
Applications: WB, Flow, ICC/IF, IHC-Fr, IHC-P, IP, PAGE
NBP1-90306
Species: Hu
Applications: WB, IHC, IHC-P
NBP1-59069
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P
NBL1-11228
Species: Hu
NBP1-31293
Species: Hu, Mu
Applications: WB, ICC/IF
NBP2-38770
Species: Hu, Mu
Applications: WB, IHC, IHC-P
NBP2-36776
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
AF682
Species: Hu
Applications: WB, IHC, ELISA(Cap), ELISA(Det), Neut, ELISA(Sta)
NBP1-30475
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P, IP, PLA
NBP2-47645
Species: Hu, Mu, Rt
Applications: Flow, IHC-P, PAGE, IF

Publications for Elastase Antibody (NBP2-47645) (0)

There are no publications for Elastase Antibody (NBP2-47645).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for Elastase Antibody (NBP2-47645) (0)

There are no reviews for Elastase Antibody (NBP2-47645). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for Elastase Antibody (NBP2-47645) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional Elastase Products

Bioinformatics Tool for Elastase Antibody (NBP2-47645)

Discover related pathways, diseases and genes to Elastase Antibody (NBP2-47645). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for Elastase Antibody (NBP2-47645)

Discover more about diseases related to Elastase Antibody (NBP2-47645).
 

Pathways for Elastase Antibody (NBP2-47645)

View related products by pathway.

Research Areas for Elastase Antibody (NBP2-47645)

Find related products by research area.

Blogs on Elastase

There are no specific blogs for Elastase, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Elastase Antibody (CELA3B/1257) and receive a gift card or discount.

Bioinformatics

Gene Symbol CELA3B