ELA3A Antibody (CELA3A/12552)

• Reactivity: Hu
• Applications: WB, IHC
• Clone: CELA3A/12552
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

ELA3A Antibody (CELA3A/12552) Summary

• Description: 200ug/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml.
  
  Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. Non-hazardous. No MSDS required.
• Immunogen: Recombinant fragment (around aa50-250) of human ELA3A protein (Uniprot: P09093). Exact sequence is proprietary.
• Localization: Cytoplasm, Extracellular space
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: CELA3A
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1-2ug/ml
  • Western Blot 2-4ug/ml
• Application Notes: Immunohistochemistry: 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
• Theoretical MW: 29 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: 10 mM PBS with 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Protein G purified

Alternate Names for ELA3A Antibody (CELA3A/12552)

• chymotrypsin-like elastase family member 3A
• chymotrypsin-like elastase family, member 3A
• EC 3.4.21
• EC 3.4.21.70
• ELA3A
• ELA3elastase 1
• elastase 3A, pancreatic (protease E)
• elastase 3A, pancreatic
• Elastase IIIA
• elastase-3A
• Protease E

Background

Elastases form a subfamily of serine proteases that hydrolyze many proteins in addition to elastin. Humans have six elastase genes which encode the structurally similar proteins elastase 1, 2, 2A, 2B, 3A, and 3B. Unlike other elastases, elastase 3A has little elastolytic activity. Like most of the human elastases, elastase 3A is secreted from the pancreas as a zymogen and, like other serine proteases such as trypsin, chymotrypsin and kallikrein, it has a digestive function in the intestine. Elastase 3A preferentially cleaves proteins after alanine residues. Elastase 3A may also function in the intestinal transport and metabolism of cholesterol. Both elastase 3A and elastase 3B have been referred to as protease E and as elastase 1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CELA3A