Recombinant Human eIF4H His Protein

SDS-Page: Recombinant Human eIF4H Protein [NBP1-98965] - 15% SDS-PAGE (3ug)

• Reactivity: Hu
• Applications: PAGE
• Concentration: 0.5 mg/ml

Recombinant Human eIF4H His Protein Summary

• Description: A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-248 of Human eIF4H
  
  

  Source: E.coli

  Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSHMADFDT YDDRAYSSFG GGRGSRGSAG GHGSRSQKEL PTEPPYTAYV GNLPFNTVQG DIDAIFKDLS IRSVRLVRDK DTDKFKGFCY VEFDEVDSLK EALTYDGALL GDRSLRVDIA EGRKQDKGGF GFRKGGPDDR GMGSSRESRG GWDSRDDFNS GFRDDFLGGR GGSRPGDRRT GPPMGSRFRD GPPLRGSNMD FREPTEEERA QRPRLQLKPR TVATPLNQVA NPNSAIFGGA RPREEVVQKE QE

• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: EIF4H
• Purity: >90%, by SDS-PAGE

Applications/Dilutions

• Dilutions:
  • SDS-Page
• Theoretical MW: 29.9 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 20 mM Tris-HCl buffer (pH 8.0), 0.2 M NaCl, 50% glycerol, 2 mM DTT
• Preservative: No Preservative
• Concentration: 0.5 mg/ml
• Purity: >90%, by SDS-PAGE

Alternate Names for Recombinant Human eIF4H His Protein

• eukaryotic translation initiation factor 4H
• KIAA0038eIF-4H
• WBSCR1
• Williams-Beuren syndrome chromosome region 1
• WSCR1Williams-Beuren syndrome chromosomal region 1 protein

Background

Eukaryotic translation initiation factor 4H, also known as EIF4H, is a 248 amino acid protein that localizes to the perinuclear region of the cytoplasm and is expressed as two isoforms, designated short and long. EIF4H induces the RNA-dependent ATP hydrolysis catalyzed by the initiation factors EIF4A and EIF4B. EIF4H was further shown to stimulate the initial rate and extent of EIF4A-mediated mRNA secondary structure unwinding. Defects in the gene encoding EIF4H are associated with Williams- Beuren syndrome (WBS), a rare developmental disorder characterized by cardiovascular and musculo-skeletal abnormalities. Recombinant human EIF4H protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: EIF4H