EIF4A3 Antibody (S05-1B7)

Western Blot: EIF4A3 Antibody (S05-1B7) [NBP3-14928] - Western blot detection of EIF4A3 in Hela, CHO-K1, C6 cell lysates using EIF4A3 Rabbit mAb (1:500 diluted). Predicted band size: 47kDa. Observed band size: 47kDa.

Immunohistochemistry-Paraffin: EIF4A3 Antibody (S05-1B7) [NBP3-14928] - Immunohistochemistry of EIF4A3 in paraffin-embedded Human tonsil using EIF4A3 Rabbit mAb at dilution 1/50

Immunocytochemistry/Immunofluorescence: EIF4A3 Antibody (S05-1B7) [NBP3-14928] - Immunofluorescence of EIF4A3 (green) in Hela using NBP3-14928 at dilution 1/5, and DAPI (blue)

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC
• Clone: S05-1B7
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated

EIF4A3 Antibody (S05-1B7) Summary

• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: A synthetic peptide of human EIF4A3 (Uniprot#: P38919)
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: EIF4A3
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1/100
  • Immunohistochemistry 1/20
  • Immunohistochemistry-Paraffin
  • Western Blot 1/1000
• Theoretical MW: 47 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 40% Glycerol, 0.05% BSA
• Preservative: 0.01% Sodium Azide
• Purity: Affinity purified

Alternate Names for EIF4A3 Antibody (S05-1B7)

• ATP-dependent RNA helicase DDX48
• ATP-dependent RNA helicase eIF4A-3
• DDX48
• DEAD (Asp-Glu-Ala-Asp) box polypeptide 48
• DEAD box protein 48
• DKFZp686O16189
• EC 3.6.1
• EC 3.6.4.13
• EIF4AIII
• eIF-4A-III
• eIF4A-III
• eukaryotic initiation factor 4A-III
• Eukaryotic initiation factor 4A-like NUK-34
• Eukaryotic translation initiation factor 4A isoform 3
• eukaryotic translation initiation factor 4A
• eukaryotic translation initiation factor 4A3
• hNMP 265
• KIAA0111eukaryotic translation initiation factor 4A, isoform 3
• MGC10862
• NMP 265
• NMP265
• Nuclear matrix protein 265
• NUK34

Background

EIF4A3 encodes a member of the DEAD box protein family. DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. The protein encoded by this gene is a nuclear matrix protein. Its amino acid sequence is highly similar to the amino acid sequences of the translation initiation factors eIF4AI and eIF4AII, two other members of the DEAD box protein family.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: EIF4A3