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eIF3X Antibody

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Western Blot: eIF3X Antibody [NBP3-03247] - Analysis of extracts of various cell lines, using eIF3x antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit.
Western Blot: eIF3X Antibody [NBP3-03247] - Analysis of extracts of various cell lines, using eIF3x antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: ...read more
Immunocytochemistry/ Immunofluorescence: eIF3X Antibody [NBP3-03247] - Analysis of U-2 OS cells using eIF3x antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunohistochemistry-Paraffin: eIF3X Antibody [NBP3-03247] - Paraffin-embedded mouse heart using eIF3x antibody at dilution of 1:100 (40x lens).
Immunocytochemistry/ Immunofluorescence: eIF3X Antibody [NBP3-03247] - Analysis of NIH-3T3 cells using eIF3x antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunohistochemistry-Paraffin: eIF3X Antibody [NBP3-03247] - Paraffin-embedded human placenta using eIF3x antibody at dilution of 1:100 (40x lens).
Immunohistochemistry-Paraffin: eIF3X Antibody [NBP3-03247] - Paraffin-embedded human stomach using eIF3x antibody at dilution of 1:100 (40x lens).

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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eIF3X Antibody Summary

Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1120-1309 of human eIF3X (NP_056044.3).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CLUH
Purity
Affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • Immunocytochemistry/Immunofluorescence 1:50-1:200
  • Immunohistochemistry 1:50-1:200
  • Immunohistochemistry-Paraffin
  • Western Blot 1:1000-1:4000
Theoretical MW
146 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS with 50% glycerol, pH7.3.
Preservative
0.02% Sodium Azide
Purity
Affinity purified

Alternate Names for eIF3X Antibody

  • CLU1
  • DKFZp686L05242
  • DKFZp686M09233
  • FLJ23672
  • hypothetical protein LOC23277
  • KIAA0664

Background

eIF3X (putative eukaryotic translation initiation factor 3 subunit) belongs to the eIF-3 p135 family of evolutionarily conserved eIF-3 135kDa subunits found in yeast, nematode, slime mold, and human cells. In yeast, eIF3 p135 is not part of the core eIF-3 complex, but is proposed to be a probable component with unknown function

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol CLUH