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EIF3C Antibody [HRP]

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Product Details

Summary
Reactivity Hu, Mu, Rt, Bv, Pm-OrSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Rabbit
Conjugate
HRP

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NB100-511AF350
Alexa Fluor 405
NB100-511AF405
Alexa Fluor 488
NB100-511AF488
Alexa Fluor 532
NB100-511AF532
Alexa Fluor 594
NB100-511AF594
Alexa Fluor 647
NB100-511AF647
Alexa Fluor 700
NB100-511AF700
Alexa Fluor 750
NB100-511AF750
Biotin
NB100-511B
DyLight 350
NB100-511UV
DyLight 405
NB100-511V
DyLight 488
NB100-511G
DyLight 550
NB100-511R
DyLight 594
NB100-511DL594
DyLight 650
NB100-511C
DyLight 680
NB100-511FR
DyLight 755
NB100-511IR
Unconjugated
NB100-511
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EIF3C Antibody [HRP] Summary

Immunogen
A synthetic peptide derived from a sequence between residues 850 and 918 (the C-terminus) of human eukaryotic translation initiation factor 3, subunit 8, 110kDa using the numbering given in Swiss-Prot entry Q99613 (GeneID 8663).
Predicted Species
Rat (100%), Bovine (100%), Orangutan (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
EIF3C
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

Mouse reactivity reported in (PMID: 28241004).

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
PBS
Preservative
No Preservative
Purity
Immunogen affinity purified

Notes

This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for EIF3C Antibody [HRP]

  • cell migration-inducing protein 17
  • eIF3 p110
  • eIF3c
  • EIF3CL
  • eIF3-p110
  • EIF3S8FLJ55450
  • Eukaryotic translation initiation factor 3 subunit 8
  • eukaryotic translation initiation factor 3 subunit C
  • eukaryotic translation initiation factor 3, subunit 8 (110kD)
  • eukaryotic translation initiation factor 3, subunit 8, 110kDa
  • eukaryotic translation initiation factor 3, subunit C
  • FLJ53378
  • FLJ54400
  • FLJ54404
  • FLJ55750
  • FLJ78287
  • MGC189737
  • MGC189744

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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EIF5 Antibody
NBP1-86184
Western Blot: EIF5 Antibody [NBP1-86184] - Analysis in U2OS cells transfected with control siRNA, target specific siRNA probe #1 and #2. Remaining relative intensity is presented.
Immunocytochemistry/Immunofluorescence: EIF5 Antibody [NBP1-86184] - Staining of human cell line U-2 OS shows positivity in cytoplasm.
Immunohistochemistry-Paraffin: EIF5 Antibody [NBP1-86184] - Staining of human stomach shows strong cytoplasmic and membranous positivity in glandular cells.
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P, KD
Eukaryotic
translation initiatio ...
NBP2-00702
Western Blot: Eukaryotic translation initiation factor 1 Antibody (2B9) [NBP2-00702] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Eukaryotic translation initiation factor 1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Eukaryotic translation initiation factor 1.
Immunocytochemistry/Immunofluorescence: Eukaryotic translation initiation factor 1 Antibody (2B9) [NBP2-00702] Staining of COS7 cells transiently transfected by pCMV6-ENTRY Eukaryotic translation initiation factor 1.
Immunohistochemistry-Paraffin: Eukaryotic translation initiation factor 1 Antibody (2B9) [NBP2-00702] - Staining of paraffin-embedded Human lymph node tissue using anti-Eukaryotic translation initiation factor 1 mouse monoclonal antibody.
Species: Hu
Applications: WB, Flow, ICC/IF, IHC, IHC-P
EIF3B Antibody
NBP2-24571
Western Blot: EIF3B Antibody [NBP2-24571] - analysis of eIF3B in A431 cell lysate in the 1) absence and 2) presence of immunizing peptide and 3) NIH 3T3 cell lysate using eIF3B antibody at 0.25 ug/ml, 0.25 ug/ml and 0.05 ug/ml respectively.
Immunohistochemistry-Paraffin: EIF3B Antibody [NBP2-24571] - Formalin-fixed, paraffin-embedded human brain tissue stained with eIF3B antibody at 10 ug/ml. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.
Simple Western: EIF3B Antibody [NBP2-24571] - Simple Western lane view shows a specific band for EIF3B in 0.5 mg/ml of A431 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.
Species: Hu, Mu
Applications: WB, Simple Western, IHC, IHC-P
EIF3A Antibody
NBP2-24570
Western Blot: EIF3A Antibody [NBP2-24570] - analysis of eIF3A in Daudi lysate in the 1) absence and 2) presence of immunizing peptide and 3) NIH 3T3 lysate using eIF3A antibody at 0.5 ug/ml, 0.5 ug/ml and 0.25 ug/ml respectively.
Immunohistochemistry-Paraffin: EIF3A Antibody [NBP2-24570] - Formalin-fixed, paraffin-embedded human brain tissue stained with eIF3A antibody at 10 ug/ml. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.
Simple Western: EIF3A Antibody [NBP2-24570] - Simple Western lane view shows a specific band for EIF3A in 0.5 mg/ml of NIH-3T3 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.
Species: Hu, Mu
Applications: WB, Simple Western, IHC, IHC-P
integrin beta 4
binding protein Antib ...
NBP2-13954
Western Blot: integrin beta 4 binding protein Antibody [NBP2-13954] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).
Immunocytochemistry/Immunofluorescence: integrin beta 4 binding protein Antibody [NBP2-13954] - Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.
Immunohistochemistry-Paraffin: integrin beta 4 binding protein Antibody [NBP2-13954] - Staining of human skeletal muscle shows low expression as expected.
(1 Publication)
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
CBP20 Antibody
NBP1-91755
Western Blot: CBP20 Antibody [NBP1-91755] - Analysis in control (vector only transfected HEK293T lysate) and NCBP2 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunocytochemistry/Immunofluorescence: CBP20 Antibody [NBP1-91755] - Staining of human cell line U-2 OS shows localization to nucleoplasm. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: CBP20 Antibody [NBP1-91755] - Staining of human testis shows moderate nuclear positivity in cells in seminiferous ducts.
(1 Publication)
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
BNIP1 Antibody
(OTI2B3)
NBP2-46580
Western Blot: BNIP1 Antibody (2B3) [NBP2-46580] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BNIP1.
Immunohistochemistry: BNIP1 Antibody (2B3) [NBP2-46580] - Analysis of Human prostate tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunohistochemistry: BNIP1 Antibody (2B3) [NBP2-46580] - Analysis of Human pancreas tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Species: Hu
Applications: WB, IHC
EIF2 beta Antibody
(2F3)
H00008894-M09
Western Blot: EIF2 beta Antibody (2F3) [H00008894-M09] - Analysis of EIF2S2 expression in NIH/3T3 (Cat # L018V1).
Immunocytochemistry/Immunofluorescence: EIF2 beta Antibody (2F3) [H00008894-M09] - Analysis of monoclonal antibody to EIF2S2 on HeLa cell. Antibody concentration 10 ug/ml
Immunohistochemistry-Paraffin: EIF2 beta Antibody (2F3) [H00008894-M09] - Analysis of monoclonal antibody to EIF2S2 on formalin-fixed paraffin-embedded human pancreatic cancer. Antibody concentration 3 ug/ml
Species: Hu, Mu, Rt
Applications: WB, ELISA, ICC/IF, IHC, IHC-P, S-ELISA
NECAB3 Antibody
NBP2-68925
Western Blot: NECAB3 Antibody [NBP2-68925] - Analysis in human cell line RT-4, human cell line U-251 MG, human plasma, human liver tissue and human tonsil tissue.
Immunohistochemistry: NECAB3 Antibody [NBP2-68925] - Immunohistochemical staining of human adrenal gland shows strong cytoplasmic positivity in glandular cells.
Species: Hu
Applications: WB, IHC, IHC-P
Sonic Hedgehog/Shh
[Unconjugated]
1845-SH
1 μg/lane of Recombinant Human Sonic Hedgehog/Shh (C24II), N-Terminus was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 22 kDa.
MALDI-TOF analysis of Recombinant Human Sonic Hedgehog/Shh (C24II), N-Terminus. The major peak corresponds to the calculated molecular mass, 19814 Da. The minor peak at 20029  is a matrix-associated artifact of the MALDI-TOF.
Recombinant Human Sonic Hedgehog/Shh (C24II), N-Terminus (Catalog # 1845-SH) induces alkaline phosphatase production by the C3H10T1/2 mouse embryonic fibroblast cell line. The ED50 for this effect is 0.1‑0.4 μg/mL.
(5 Reviews)
(37 Publications)
Species: Hu
NF2/Merlin Antibody
NBP1-87757
Western Blot: NF2/Merlin Antibody [NBP1-87757] - Analysis in U2OS cells transfected with control siRNA, target specific siRNA probe #1 and #2,. Remaining relative intensity is presented. Loading control: Anti-GAPDH.
Immunocytochemistry/Immunofluorescence: NF2/Merlin Antibody [NBP1-87757] - Staining of human cell line U-251 MG shows localization to plasma membrane & cytosol. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: NF2/Merlin Antibody [NBP1-87757] - Staining of human fallopian tube shows moderate cytoplasmic and moderate membranous positivity in glandular cells.
(3 Publications)
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P, IP, KD
FUZ Antibody
NBP2-24750
Western Blot: FUZ Antibody [NBP2-24750] - analysis of FUZ in human lung lysate in the 1) absence and 2) presence of immunizing peptide, 3) mouse lung and 4) rat lung using this antibody. 0 ug/ml, 2.0 ug/ml and 6.0 ug/ml respectively.
Immunohistochemistry-Paraffin: FUZ Antibody [NBP2-24750] - Formalin-fixed, paraffin-embedded human lung stained with FUZ antibody at 15 ug/ml. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.
Species: Hu, Mu, Rt, Pm
Applications: WB, IHC, IHC-P
eIF3e Antibody
NBP1-84869
Western Blot: eIF3e Antibody [NBP1-84869] - Analysis in A-431 cells transfected with control siRNA, target specific siRNA probe #1 and #2,. Remaining relative intensity is presented. Loading control: Anti-GAPDH.
Immunocytochemistry/Immunofluorescence: eIF3e Antibody [NBP1-84869] - Staining of human cell line U-2 OS shows localization to cytosol. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: eIF3e Antibody [NBP1-84869] - Staining of human pancreas shows moderate cytoplasmic positivity in exocrine cells.
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P, KD
EIF3G Antibody
NBP2-24524
Western Blot: EIF3G Antibody [NBP2-24524] - analysis of eIF3G in Ramos cell lysate in the 1) absence and 2) presence of immunizing peptide and 3) RAW cell lysate using this antibody. 025 ug/ml. I goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.
Immunohistochemistry-Paraffin: EIF3G Antibody [NBP2-24524] - Formalin-fixed, paraffin-embedded human kidney tissue stained with eIF3G antibody at 10 ug/ml. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.
Species: Hu, Mu, Rt, Bv, Ca, Eq, Pm
Applications: WB, IHC, IHC-P
eIF4G1 Antibody
NB100-268
Western Blot: eIF4G1 Antibody [NB100-268] - Whole cell lysate (50 ug) from HeLa and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-eiF4G1/eiF4GI antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Immunoprecipitation: eIF4G1 Antibody [NB100-268] - Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-eiF4G1/eiF4GI antibody used for IP at 6 ug per reaction. eiF4G1/eiF4GI was also immunoprecipitated by rabbit antieiF4G1/eiF4GI antibody. For blotting immunoprecipitated eiF4G1/eiF4GI, rabbit antieiF4G1/eiF4GI antibody was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
(24 Publications)
Species: Hu, Po
Applications: WB, IP
GLI-3 Antibody
AF3690
Western blot shows lysates of mouse embryo tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse GLI‑3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3690) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for GLI‑3 at approximately 85 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Jurkat human acute T cell leukemia cell line treated with 50 ng/mL PMA and 200 ng/mL calcium ionomycin for 30 minutes was fixed using formaldehyde, resuspended in lysis buffer, and sonicated to shear chromatin. GLI‑3/DNA complexes were immunoprecipitated using 5 μg Goat Anti-Human/Mouse GLI‑3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3690) or control antibody (Catalog # AB-108-C) for 15 minutes in an ultrasonic bath, followed by Biotinylated Anti-Goat IgG Secondary Antibody (Catalog # BAF109). Immunocomplexes were captured using 50 μL of MagCellect Streptavidin Ferrofluid (Catalog # MAG999) and DNA was purified using chelating resin solution. The gli-1 promoter was detected by standard PCR.
GLI‑3 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Human/Mouse GLI‑3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3690) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (yellow; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
(5 Reviews)
(35 Publications)
Species: Hu, Mu
Applications: WB, ChIP, ICC
MED28 Antibody
NBP1-85104
Western Blot: MED28 Antibody [NBP1-85104] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).
Immunohistochemistry-Paraffin: MED28 Antibody [NBP1-85104] - Staining of human placenta shows nuclear positivity in trophoblastic cells.
Western Blot: MED28 Antibody [NBP1-85104] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp
Species: Hu, Mu, Rt
Applications: WB, IHC, IHC-P
COPS8 Antibody
H00010920-B01P
Western Blot: COPS8 Antibody [H00010920-B01P] - Analysis of COPS8 expression in rat brain.
Immunocytochemistry/Immunofluorescence: COPS8 Antibody [H00010920-B01P] - Analysis of purified antibody to COPS8 on HeLa cell. (antibody concentration 10 ug/ml)
Western Blot: COPS8 Antibody [H00010920-B01P] - analysis of COPS8 expression in transfected 293T cell line by COPS8 MaxPab polyclonal antibody. Lane 1: COPS8 transfected lysate (23.1 KDa). Lane 2: Non-transfected lysate.
Species: Hu, Rt
Applications: WB, ICC/IF

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NB100-511AF350
Alexa Fluor 405 NB100-511AF405
Alexa Fluor 488 NB100-511AF488
Alexa Fluor 532 NB100-511AF532
Alexa Fluor 594 NB100-511AF594
Alexa Fluor 647 NB100-511AF647
Alexa Fluor 700 NB100-511AF700
Alexa Fluor 750 NB100-511AF750
Biotin NB100-511B
DyLight 350 NB100-511UV
DyLight 405 NB100-511V
DyLight 488 NB100-511G
DyLight 550 NB100-511R
DyLight 594 NB100-511DL594
DyLight 650 NB100-511C
DyLight 680 NB100-511FR
DyLight 755 NB100-511IR
HRP NB100-511H
Learn about Custom Antibody Labeling

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Bioinformatics

Gene Symbol EIF3C
Entrez
Uniprot